Merck
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S4019

Sigma-Aldrich

Sodium bicarbonate

Hybri-Max, powder, suitable for hybridoma, ≥99.5%

Synonym(s):
Sodium hydrogen carbonate
Linear Formula:
NaHCO3
CAS Number:
Molecular Weight:
84.01
Beilstein:
4153970
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.75

grade

Hybri-Max

Quality Level

assay

≥99.5%

form

powder

technique(s)

cell culture | hybridoma: suitable

impurities

endotoxin, tested

pH

8.3 (0.1 molfreshly prepared)

pKa (25 °C)

(1) 6.37, (2) 10.25 (carbonic acid)

solubility

water: soluble 100 mg/mL

density

2.16 g/mL at 25 °C (lit.)

SMILES string

[Na+].OC([O-])=O

InChI

1S/CH2O3.Na/c2-1(3)4;/h(H2,2,3,4);/q;+1/p-1

InChI key

UIIMBOGNXHQVGW-UHFFFAOYSA-M

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Application

Sodium bicarbonate has been used as a supplement:
  • in minimum essential medium (MEM)/flushing medium for embryo isolation
  • in TCM 199 medium for the collection of cumulus-oocyte complexes (COCs) and in vitro maturation (IVM)
  • in IVM medium for in vitro maturation of COCs

Packaging

500 g in poly bottle
1 kg in poly bottle

Biochem/physiol Actions

Sodium bicarbonate occurs naturally as the mineral nahcolite. It is used to prepare many sodium salts as well as a carbon dioxide source. Sodium bicarbonate is used in the manufacture of fire extinguishers and cleaning agents. Bicarbonate plays an important role in pH regulation in vivo. In biochemistry, molecular biology, and chromatography, sodium bicarbonate is used as a buffer. This Hybri-Max product is suitable for use in hybridoma and cell culture research (2.2 mg/ml).

Legal Information

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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