SAB4200811

Anti- Neurofilament 200-FITC antibody, Mouse monoclonal

clone NE14, purified from hybridoma cell culture

• Synonym(s): H-subunit, NF-H

Properties

• biological source: mouse
• conjugate: FITC conjugate
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: NE14, monoclonal
• form: buffered aqueous solution
• mol wt: 200 kDa
• species reactivity: pig, feline, chicken, bovine, human, mouse, guinea pig, rat
• packaging: antibody small pack of 25 μL
• concentration: ~1 mg/mL
• technique(s): immunohistochemistry: 1:200-1:400 (4-8 ug/mL) using enzyme treated formalin-fixed, paraffin-embedded rat Cerebellum sections
• shipped in: dry ice
• storage temp.: −20°C
• Gene Information: human ... NEFH(4744)

Description

General description

Neurofilaments are type of intermediate filaments (IFs) that serve as major elements of the cytoskeleton supporting the axon cytoplasm of neuronal cells. IFs are components of most eukaryotic cells and significantly differ from other cytoskeletal elements of the cell, namely microtubules and microfilaments.

Specificity

Monoclonal Anti-Neurofilament 200, also known as Neurofilament-H or Heavy subunit, specifically recognizes the phosphorylated H tail of Neurofilament 200 and shows no reactivity on enzymatically dephosphorylated neurofilaments.2 The antibody shows reactivity with neurofilaments in the central and peripheral nervous systems from human1, pig1, mouse3, rat4, chicken3, guinea pig3, feline3 and bovine3 origin.

Immunogen

Neurofilaments purified from pig spinal cord

Application

The antibody may be used in various immunochemical techniques including Immunohistochemistry and Immunoblotting (~200 kDa).1-7

Biochem/physiol Actions

Neurofilaments undergo post-translational modifications including different levels of phosphorylation, which has been suggested to modulate their function by influencing the interaction between neurofilament and cytoplasmic organelles. Neurofilaments are built from three intertwined protofibrils of apparent molecular weights [68 (L), 160 (M) and 200 (H) kDa] which are themselves composed of two tetrameric protofilament complexes of monomeric proteins. Neurofilament 200 also known as Neurofilament heavy polypeptide (H-subunit), NF-H, NEFH or 200 kDa neurofilament protein, has an important function in mature axons that is not subserved by the two smaller neurofilament proteins.

Physical form

Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.

Storage and Stability

For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours. Protect from prolonged exposure to light.

Disclaimer

Unless otherwise stated in our catalog  our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable