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SAB5500058

Sigma-Aldrich

Anti-CD3 antibody, Rabbit monoclonal

clone SP7, recombinant, expressed in proprietary host, tissue culture supernatant

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Synonym(s):
Anti-CD3epsilon, Anti-IMD18, Anti-T3E, Anti-TCRE
NACRES:
NA.41

biological source

rabbit

Quality Level

recombinant

expressed in proprietary host

conjugate

unconjugated

antibody form

tissue culture supernatant

antibody product type

primary antibodies

clone

SP7, monoclonal

species reactivity

human (tested)

species reactivity (predicted by homology)

pig, rat, dog, bovine, rabbit, chicken

technique(s)

flow cytometry: 1:100
immunoblotting: 1:25
immunohistochemistry: 1:150

isotype

IgG

UniProt accession no.

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... CD3E(916)

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recombinant

expressed in proprietary host

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recombinant

expressed in proprietary host

recombinant

expressed in proprietary host

conjugate

unconjugated

conjugate

-

conjugate

unconjugated

conjugate

unconjugated

antibody form

tissue culture supernatant

antibody form

purified immunoglobulin

antibody form

tissue culture supernatant

antibody form

tissue culture supernatant

clone

SP7, monoclonal

clone

RM344, monoclonal, recombinant monoclonal

clone

SP23, monoclonal

clone

SP16, monoclonal

species reactivity

human (tested)

species reactivity

human

species reactivity

human (tested)

species reactivity

human (tested)

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General description

This antibody reacts with the intracytoplasmic portion of the CD3 antigen expressed by T cells. It stains human T cells in both the cortex and medulla of the thymus and in peripheral lymphoid tissues. This antibody is suitable for staining normal and neoplastic T cells in formalin-fixed, paraffin-embedded tissues.

Immunogen

Synthetic peptide corresponding to internal region of the epsilon chain of human CD3 protein.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

0.1 ml rabbit monoclonal antibody supplied as tissue culture supernatant in TBS/1% BSA buffer pH 7.5 with less than 0.1% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Vikrant Rai et al.
Scientific reports, 10(1), 15167-15167 (2020-09-18)
Cells of the immune system are present in the adult cochlea and respond to damage caused by noise exposure. However, the types of immune cells involved and their locations within the cochlea are unclear. We used flow cytometry and immunostaining
Yongfeng He et al.
Cell reports. Medicine, 2(9), 100392-100392 (2021-10-09)
Thymic carcinoma is rare and has a poorer prognosis than thymomas. The treatment options are limited after failure of platinum-based chemotherapy. We previously performed a single-center phase II study of pembrolizumab in patients with advanced thymic carcinoma, showing a 22.5%
Paul D Simonson et al.
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Multiparametric imaging allows researchers to measure the expression of many biomarkers simultaneously, allowing detailed characterization of cell microenvironments. One such technique, CODEX, allows fluorescence imaging of >30 proteins in a single tissue section. In the commercial CODEX system, primary antibodies
Filippos T Charitidis et al.
In vivo (Athens, Greece), 35(4), 1985-1997 (2021-06-29)
The pathway of initiation of psoriasis comprises the differentiation and infiltration of T-helper 17 (Th17) cells into the skin, characterized by the production of interleukin 17A and 17F (IL-17A/IL-17F) among other cytokines, resulting in a downstream cascade of events. Due
Xue Wu et al.
Cancer science, 112(11), 4444-4456 (2021-08-13)
WEE1 plays an important role in the regulation of cell cycle G2/M checkpoints and DNA damage response (DDR). Inhibition of WEE1 can increase the instability of the genome and have anti-tumor effects in some solid tumors. However, it has certain

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