Merck
All Photos(3)

Documents

SAB5600101

Sigma-Aldrich

Anti-LC3B antibody, Rabbit Monoclonal

recombinant, expressed in HEK 293 cells, clone RM293, purified immunoglobulin

Synonym(s):
Autophagy-related protein LC3 B
NACRES:
NA.41

recombinant

expressed in HEK 293 cells

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

RM293, monoclonal
recombinant monoclonal

form

buffered aqueous glycerol solution

species reactivity

human

technique(s)

immunocytochemistry: 1:100-1:200
immunohistochemistry: 1:100-1:200
western blot: 1:100-1:200

isotype

IgG

NCBI accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
SAB4200846SAB4200361SAB5600151
antibody form

purified immunoglobulin

antibody form

affinity isolated antibody, purified from hybridoma cell culture

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

clone

RM293, monoclonal, recombinant monoclonal

clone

LC3C-37, monoclonal

clone

LC3B-6, monoclonal

clone

RM344, monoclonal, recombinant monoclonal

form

buffered aqueous glycerol solution

form

-

form

buffered aqueous solution

form

buffered aqueous glycerol solution

species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

human

technique(s)

immunocytochemistry: 1:100-1:200, immunohistochemistry: 1:100-1:200, western blot: 1:100-1:200

technique(s)

immunoblotting: 1-2 μg/mL using whole extracts of HEK-293T cells overexpressing human LC3C., immunofluorescence: 2.5-5 μg/mL using human HeLa cells treated with Bafilomycin A1 for autophagosomes accumulation

technique(s)

immunoprecipitation (IP): 10-20 μg using lysates of human U-87 cells., western blot: 1-2 μg/mL using whole extracts of HEK-293T cells over-expressing human LC3B.

technique(s)

immunohistochemistry: 1:100-1:200

Specificity

This antibody reacts to human LC3B (Autophagy-related protein LC3 B) (Microtubule-associated proteins 1A/1B light chain 3B). It may also react to mouse or rat LC3B, as predicted by immunogen homology.

Immunogen

A peptide corresponding to the N-terminus of human LC3B.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Solution in phosphate buffered saline containing 50% glycerol, 1% BSA and 0.09% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (Example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Sifan Gao et al.
Technology in cancer research & treatment, 19, 1533033820970664-1533033820970664 (2020-11-13)
The objective of this study was to investigate the between autophagy activity and local immune response in hypopharyngeal squamous cell carcinoma (HSCC). Herein, we observed the expression of autophagy marker microtubule-associated protein light chain 3B (MAP1LC3B), CD8 cytotoxic T lymphocytes

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service