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SAB5600104

Sigma-Aldrich

Anti-Vimentin antibody, Rabbit Monoclonal

recombinant, expressed in HEK 293 cells, clone RM289, purified immunoglobulin

NACRES:
NA.41

recombinant

expressed in HEK 293 cells

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

RM289, monoclonal
recombinant monoclonal

form

buffered aqueous glycerol solution

species reactivity

human

technique(s)

immunohistochemistry: 1:100-1:200
western blot: 1:200-1:500

isotype

IgG

NCBI accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... VIM(7431)

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This Item
SAB5500191SAB4200761SAB4200716
antibody form

purified immunoglobulin

antibody form

tissue culture supernatant

antibody form

purified from hybridoma cell culture

antibody form

purified from hybridoma cell culture

clone

RM289, monoclonal, recombinant monoclonal

clone

SP20, monoclonal

clone

LN-6, monoclonal

clone

VIM-13.2, monoclonal

form

buffered aqueous glycerol solution

form

-

form

buffered aqueous solution

form

buffered aqueous solution

species reactivity

human

species reactivity

human (tested)

species reactivity

rat, human, porcine, monkey, bovine, canine, mouse, rabbit, hamster

species reactivity

mouse, bovine, canine, rat, monkey, human, chicken

technique(s)

immunohistochemistry: 1:100-1:200, western blot: 1:200-1:500

technique(s)

flow cytometry: 1:100, immunoblotting: 1:100, immunohistochemistry: 1:200

technique(s)

flow cytometry: suitable, immunoblotting: 0.25-0.5 μg/mL using human foreskin fibroblast Hs68 cell line., immunofluorescence: 1-2 μg/mL using human foreskin fibroblast Hs68 cell line., immunohistochemistry: 5-10 μg/mL using Human breast carcinoma sections., immunoprecipitation (IP): suitable

technique(s)

immunoblotting: 0.25-0.5 μg/mL using human foreskin fibroblast HS68 cell line extract, immunofluorescence: 1.25-2.5 μg/mL using human foreskin fibroblast HS68 cells, immunohistochemistry: suitable

Specificity

This antibody reacts to human Vimentin. It may also react to mouse or rat Vimentin, as predicted by immunogen homology.

Immunogen

A peptide corresponding to the C-terminus of human Vimentin.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Solution in phosphate buffered saline containing 50% glycerol, 1% BSA and 0.09% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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