U5378

Urea

powder, BioReagent, Molecular Biology, suitable for cell culture

• Synonym(s): Carbamide, Carbonyldiamide
• Linear Formula: NH₂CONH₂
• CAS Number: 57-13-6
• Molecular Weight: 60.06
• Beilstein: 635724
• EC Number: 200-315-5
• MDL number: MFCD00008022
• UNSPSC Code: 12352111
• PubChem Substance ID: 24900656
• NACRES: NA.31
• grade: Molecular Biology
• technique(s): cell culture | mammalian: suitable

Properties

• grade: Molecular Biology
• Quality Level: 200
• product line: BioReagent
• Assay: ≥98%
• form: powder
• technique(s): cell culture | mammalian: suitable
• mp: 132-135 °C (lit.)
• solubility: H₂O: soluble 480 mg/mL, clear, colorless (8M)
• density: 1.335 g/mL at 25 °C (lit.)
• anion traces: chloride (Cl^-): ≤5 ppm
• cation traces: Cu: ≤1 ppm; Fe: ≤5 ppm; Pb: ≤2 ppm
• absorption: ≤0.06 at 260 at 6 M; ≤0.06 at 280 at 6 M
• foreign activity: DNase, NICKase, RNase and protease, none detected
• SMILES string: NC(N)=O
• InChI: 1S/CH4N2O/c2-1(3)4/h(H4,2,3,4)
• InChI key: XSQUKJJJFZCRTK-UHFFFAOYSA-N
• Gene Information: human ... CA1(759), CA2(760) ; rat ... Ppm1a(24666)

Description

Application

• Urea has been used in protein resuspension buffer and to denature proteins.[1]
• It has been used for the isolation of proteins via urea-containing polyacrylamide gels.[2]
• It has been used to study the conformation of unfolded proteins under different concentrations of urea and different pH.[3]

Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves