ZRB1460

Sigma-Aldrich

Anti-HCF-1 Antibody, clone 1B17 ZooMAb® Rabbit Monoclonal

newenhanced validation

recombinant, expressed in HEK 293 cells

Quality Level

biological source

rabbit

recombinant

expressed in HEK 293 cells

antibody form

purified antibody

antibody product type

primary antibodies

clone

1B17, recombinant monoclonal

description

recombinant, expressed in HEK 293 cells

product line

ZooMAb® learn more

form

lyophilized

mol wt

calculated mol wt 208.73 kDa
observed mol wt ~70 to 210 kDa

species reactivity

rat, mouse, human

packaging

antibody small pack of 25 μL

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

application(s)

affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

isotype

IgG

epitope sequence

C-terminus

conjugate

unconjugated

Protein ID accession no.

UniProt accession no.

shipped in

ambient

storage temp.

2-8°C

General description

ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Specificity

Clone 1B17 is a ZooMAb® rabbit recombinant monoclonal antibody that detects Host cell factor 1. It targets an epitope within 23 amino acids from the C-terminal, chain-6 region. It detects all C-terminal cleavage chains.

Immunogen

KLH-conjugated linear peptide corresponding to 23 amino acids from the C-terminal, chain-6 region of human Host cell factor 1 (HCF-1).

Application

Anti-HCF-1, clone 1B17 ZooMAb®, Cat. No. ZRB1460, is a recombinant Rabbit monoclonal antibody that targets HCF-1 and is tested for use in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Quality Control Testing

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected HCF-1 in HeLa cell lysate.

Tested applications

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected HCF-1 in lysates from rat brain, A549 cells and NIH3T3 cells.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected HCF-1 in human colon tissue sections.

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected HCF-1 in NIH3T3 cells.

Affinity Binding Assay: A representative lot of this antibody bound HCFC1 with a KD of 6.1 x 10-7 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Target description

Host cell factor 1 (UniProt: P51610; also known as HCF, HCF-1, C1 factor, CFF, VCAF, VP16 accessory protein) is encoded by the HCFC1 (also known as HCF1, HFC1) gene (Gene ID: 3054) in human. HCF-1, a cellular transcription factor and co-activator that is required for progression through the G1 phase of the cell cycle. It is a heterodimeric complex that contains six N-terminal and six C-terminal chains that remain tightly associated in a non-covalent manner. It also contains eight HCF repeats that are specific proteolytic cleavage signals. This complex is proteolytically cleaved into one or several PPCE THET sites within the HCF repeats. HCF-1 does not bind to DNA directly, however, both N- and C-terminal chains are shown to associate with DNA. The Kelch domains in the N-terminal region recognize and associate with HCF-binding motif present in many sequence-specific DNA-binding factors. HCF-1 is reported to act in concert with E2F1 to activate transcription of S phase genes. It recruits KMT2E/MLL5 to E2F1 responsive promoters that facilitate transcriptional activation and G1 to S phase transition. HCF-1 is highly expressed in fetal tissues and the adult kidney. However, its expression is reported in many tested tissues. Mutations in HCFC1 gene have been linked to X-linked 3 mental retardation. In addition, a single point mutation in the Kelch domain (P134S) is reported to cause a temperature-induced cell-proliferation arrest and cytokinesis defects. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Zhou, P., et al. (2013). J. Biol. Chem. 288(24); 17532-17543; Zargar, ZU, and Tyagi, S. (2012). Transcription. 3(4); 187-192; Tyagi, S., et al. (2007). Mol. Cell. 27(1); 107-119; Vogel, JL., and Kristie, TM. (2000). Proc. Natl. Acad. Sci. USA. 97(17); 9425-9430).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

storage_class_code

11 - Combustible Solids

WGK Germany

WGK 1

Flash Point F

Not applicable

Flash Point C

Not applicable

Certificate of Analysis

Certificate of Origin

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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