DNA purification kits are used to extract and isolate DNA from samples, removing biological and chemical impurities such as cell debris, serum, proteins, lipids, and ionic components. DNA purification kits are also used to clean and prepare DNA samples for use in various downstream applications including cloning, gene editing, in situ hybridization, PCR, qPCR, multiplex PCR, sequencing, and next generation sequencing (NGS).
Classic GenElute™ DNA purification kits use a silica resin to strongly bind DNA under high salt conditions, allowing proteins, small RNAs, and other molecules to be removed through wash steps using a salt/ethanol solution. After washing, purified DNA is eluted in water or TE buffer. A broad portfolio of GenElute™ DNA purification kits tailored to specific sample types are available, enabling purification of genomic DNA (gDNA) from cell culture, mammalian tissue, blood, bacteria, viruses, plant tissue, soil, water, urine, and stool. GenElute™ Cell-Free DNA Kits provide rapid and efficient purification of circulating free DNA (cfDNA) to recover cell-free DNA fragments in the range of 100 bp – 500 bp, suitable for a wide range of downstream applications including next-generation sequencing, qPCR and bisulfite sequencing.
GenElute™-E DNA purification kits use a negative chromatography technique dependent on size exclusion to isolate and purify nucleic acid in a single spin. In size exclusion chromatography, molecules are separated by size. Smaller molecules such as proteins, lipids, and ionic components become trapped in the pores of the stationary phase of the column. Larger DNA molecules flow through the column quickly because they are too large to enter the pores. By exploiting this difference in size, GenElute™-E purification kits enable DNA purification in a single spin without the need for multiple binding and wash steps.
Extract-N-Amp™ PCR kits are the first integrated DNA extraction and amplification kits for blood, tissue, and plant samples. These innovative kits provide all the reagents necessary to rapidly release DNA and amplify targets of interest by PCR. A novel extraction method eliminates the need for long enzymatic digestions or homogenization. Kits also include a specially formulated hot start PCR ReadyMix™ for amplification directly from the extract.