Each protein preparation is unique. Give your prep the special treatment it deserves with a perfectly engineered device for dialysis or diafiltration. Our tools were designed to gently process samples while preventing precipitation or over-concentration. Find the ideal product from our selection of dialyzers and diafiltration devices that quickly and efficiently desalt, buffer exchange, and remove contaminants and impurities from protein and other biomolecular samples.
Dialyzers rely on passive diffusion for buffer exchange and desalting through placement of the dialyzer in a bath or reservoir filled with exchange buffer. Diafiltration is a technique that uses ultrafiltration for buffer exchange, eliminating salts and microsolutes in a process called “washing out”.
In both processes, separation is achieved by selective diffusion across a porous, semi-permeable membrane that allows permeation of small molecules including solvents, salts, and small metabolites while blocking the passage of larger molecules, such as proteins.
Gently and easily dialyze protein or nucleic acid samples using D-Tube™ dialyzers. These devices feature dialysis membrane windows in a capped centrifuge tube format, enabling efficient buffer exchange to remove urea, detergents, salt, and other microsolutes. Designed with a double membrane, D-Tube™ dialyzers spread the sample over a large area so that dialysis is complete in 2-5 hours.
Amicon® Ultra centrifugal filters enable rapid desalting, buffer exchange, and diafiltration of 0.5 mL -15 mL sample volumes with high sample recovery. Amicon® Ultra centrifugal devices use Ultracel® regenerated cellulose (RC) membranes for gentle ultrafiltration and are offered with nominal molecular weight limits (NMWLs) of 3 kDa, 10 kDa, 30 kDa, 50 kDa, and 100 kDa.
Stirred cells use pressurized ultrafiltration (pUF) at high flow rates to process large sample volumes for rapid diafiltration and buffer exchange. Gas pressure is applied directly to the cell for filtration.
Amicon® Stirred Cells:
Solvent-resistant stirred cells:
Disc membranes are designed for use with stirred cells for buffer exchange, desalting, and diafiltration. Ultrafiltration disc membranes are available in Ultracel® regenerated cellulose and Biomax® polyethersulvone (PES). These membranes are available in a range of different pore sizes and diameters.