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The Effective Use of Protein Kinase Inhibitors
0
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8
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84 99 99 9 64 59 20 90 93 80 94 103 96 52 55 89 82 89 99 94 102 95
86 101 105 106 34
Product Information Sheet - P5380
minutes (activity remaining):19
• 30 °C ≈ 25%
• 40 °C ≈ 40%
• 50 °C ≈ 75%
• 55-60 °C ≈ 95+%
• 65 °C ≈ 80-85%
Culturing hESCs on the Corning Synthemax Surface
Repeat D-PBS rinse.
9. Pipette cell mixture 3 to 5 times to assure single-cell suspension and dilute appropriately for
cell count (10 mL for one well of a 6 well plate; 40 mL for a T-75 flask; 120 mL for
Data Sheet - 104648
40
60
80
100
G0 G0F G1F G2F
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CHO producing IgG - D14 Glycosylation
100% Cellvento®
The Effective Use of Protein Kinase Inhibitors
www.sigma-aldrich.com/cellsignaling
84 99 99 9 64 59 20 90 93 80 94 103 96 52 55 89 82 89 99 94 102 95
86 101 105 106 34 37 57 87 81 26
Rapid Identification of coliform bacteria and E. Coli in water
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Corning Synthemax-R Surface for Research
1160
Hungary
Ingyenes telefonszám: 06 80 355 355
Ingyenes fax szám: 06 80 344 344
Tel: (+36) 1 235 9063
Fax: (+36) 1 269 6470
India
Telephone:
Bangalore: (+91) 80 6621 9400
New Delhi: (+91) 11 4358 8000
Adult Hippocampal Arctic Ground Squirrel Neural Stem Cells (AGS-NSC)
reach 70% to 80%
confluence)
12. Prepare AGS-NSC Differentiation Medium
13. Trypsinize and count cells as per instructions on page 3
14. Inoculate another Poly-L-Ornithine coated T-75 flask with
Eshmuno® CPX Resin Data Sheet
12
5
5.255.004.754.504.254.00
6
8
7
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11
120
120
120
120
120
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100
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110
120
SeQuant.Poster.HPLC_2008.HILIC_in_Clinical_Applications
Column Lifetime
Methylmalonic Acid - B -Deficiency12
0 1 2 3
Retention Time (min)
80
75
70
Acetonitrile Fraction
in Mobile Phase
K' = 2.3
K' =
Mobius®CellReady 50 L and 200 L Single-use Bioreactor Systems Performance Guide
3 4 5 6 7 8 9
0
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6
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14
Gl
uc
os
e
[g
/L
]
Culture time [d]
0 1 2 3 4
Chromatography Purification Guide
Strong 48-60 80 – 100 1-9 ++ ++ ++++
Fractogel® EMD SO3 resin
(Item #1.16882/#1.16890)
Strong 48-60, (M) type
24-40, (S) type
80 – 100 1-9 ++ +++ ++++
Montage Albumin Deplete Kit
by Radial Immunodiffusion Assay
Human Plasma Albumin
Volume (µL) Depletion (%)
20 85
50 75
75 65
100 58
Performance
Depletion of Albumin from Human Plasma
Figure 1. Human plasma
Seed Train Intensification Using High Cell Density Cryopreservation and Specially-designed Expansion Medium
400
200
0 75 61 2 3 4
Ig
G
[
m
g/
L]
days
+ 44%CHOZN® Cell Line
N=6
Expansion in Expansion Medium
Expansion in ExCEll® Advanced HD Perfusion Medium
0
Immunomonitoring using the Scepter 2.0 Cell Counter and Software Pro
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Flow Cytometry
C
ou
nt
10000 02000 4000 6000 8000 10000
R2
R1
Forward Scatter
Product Information Sheet - S1670
Range
Spin Column 10 and 30 25-50 µl 50-120 µl
Spin Column 100 40-75 µl 75-150 µl
Spin Column 200 40-75 µl 100-200 µl
Spin Column 400 40-75 µl 150-250 µl
Spin Column 1000 70-100 µl 250-350 µl
Procedure
Product Information Sheet - S0795
Range
Spin Column 10 and 30 25-50 µl 50-120 µl
Spin Column 100 40-75 µl 75-150 µl
Spin Column 200 40-75 µl 100-200 µl
Spin Column 400 40-75 µl 150-250 µl
Spin Column 1000 70-100 µl 250-350 µl
Procedure
Product Information Sheet - S2170
Range
Spin Column 10 and 30 25-50 µl 50-120 µl
Spin Column 100 40-75 µl 75-150 µl
Spin Column 200 40-75 µl 100-200 µl
Spin Column 400 40-75 µl 150-250 µl
Spin Column 1000 70-100 µl 250-350 µl
Procedure
Product Information Sheet - S1795
Range
Spin Column 10 and 30 25-50 µl 50-120 µl
Spin Column 100 40-75 µl 75-150 µl
Spin Column 200 40-75 µl 100-200 µl
Spin Column 400 40-75 µl 150-250 µl
Spin Column 1000 70-100 µl 250-350 µl
Procedure
Product Information Sheet - S0920
Range
Spin Column 10 and 30 25-50 µl 50-120 µl
Spin Column 100 40-75 µl 75-150 µl
Spin Column 200 40-75 µl 100-200 µl
Spin Column 400 40-75 µl 150-250 µl
Spin Column 1000 70-100 µl 250-350 µl
Procedure
Product Information Sheet - S1420
Range
Spin Column 10 and 30 25-50 µl 50-120 µl
Spin Column 100 40-75 µl 75-150 µl
Spin Column 200 40-75 µl 100-200 µl
Spin Column 400 40-75 µl 150-250 µl
Spin Column 1000 70-100 µl 250-350 µl
Procedure
Product Information Sheet - S1545
Range
Spin Column 10 and 30 25-50 µl 50-120 µl
Spin Column 100 40-75 µl 75-150 µl
Spin Column 200 40-75 µl 100-200 µl
Spin Column 400 40-75 µl 150-250 µl
Spin Column 1000 70-100 µl 250-350 µl
Procedure
Product Information Sheet - S1045
Range
Spin Column 10 and 30 25-50 µl 50-120 µl
Spin Column 100 40-75 µl 75-150 µl
Spin Column 200 40-75 µl 100-200 µl
Spin Column 400 40-75 µl 150-250 µl
Spin Column 1000 70-100 µl 250-350 µl
Procedure
Calcium carbonate as a replacement for titanium dioxide in coating
demonstrate
good opacity (Figure 9).
Figure 9.
CIElab values of coated tablets with Parteck® TA excipient vs. other market samples (C1–C4).
100
95
90
85
80
75
70
65
60
55
50
45
40
35
30
25
20
Product Information Sheet - S1295
Range
Spin Column 10 and 30 25-50 µl 50-120 µl
Spin Column 100 40-75 µl 75-150 µl
Spin Column 200 40-75 µl 100-200 µl
Spin Column 400 40-75 µl 150-250 µl
Spin Column 1000 70-100 µl 250-350 µl
Procedure
Product Information Sheet - S1170
Range
Spin Column 10 and 30 25-50 µl 50-120 µl
Spin Column 100 40-75 µl 75-150 µl
Spin Column 200 40-75 µl 100-200 µl
Spin Column 400 40-75 µl 150-250 µl
Spin Column 1000 70-100 µl 250-350 µl
Procedure
Product Information Sheet - S0670
Range
Spin Column 10 and 30 25-50 µl 50-120 µl
Spin Column 100 40-75 µl 75-150 µl
Spin Column 200 40-75 µl 100-200 µl
Spin Column 400 40-75 µl 150-250 µl
Spin Column 1000 70-100 µl 250-350 µl
Procedure
Product Information Sheet - S2420
Spin Column 10 and 30 25-50 µl 50-120 µl
Spin Column 100 40-75 µl 75-150 µl
Spin Column 200 40-75 µl 100-200 µl
Spin Column 400 40-75 µl 150-250 µl
Spin Column 1000 70-100 µl 250-350 µl
Procedure
Product Information Sheet - S3170
Range
Spin Column 10 and 30 25-50 µl 50-120 µl
Spin Column 100 40-75 µl 75-150 µl
Spin Column 200 40-75 µl 100-200 µl
Spin Column 400 40-75 µl 150-250 µl
Spin Column 1000 70-100 µl 250-350 µl
Procedure
Product Information Sheet - S3295
Range
Spin Column 10 and 30 25-50 µl 50-120 µl
Spin Column 100 40-75 µl 75-150 µl
Spin Column 200 40-75 µl 100-200 µl
Spin Column 400 40-75 µl 150-250 µl
Spin Column 1000 70-100 µl 250-350 µl
Procedure
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