Repeat D-PBS rinse.
9. Pipette cell mixture 3 to 5 times to assure single-cell suspension and dilute appropriately for
cell count (10 mL for one well of a 6 well plate; 40 mL for a T-75 flask; 120 mL for
reach 70% to 80%
confluence)
12. Prepare AGS-NSC Differentiation Medium
13. Trypsinize and count cells as per instructions on page 3
14. Inoculate another Poly-L-Ornithine coated T-75 flask with
by Radial Immunodiffusion Assay
Human Plasma Albumin
Volume (µL) Depletion (%)
20 85
50 75
75 65
100 58
Performance
Depletion of Albumin from Human Plasma
Figure 1. Human plasma
reproducible recovery of about 80%.
Typical recoveries with smaller and larger fragment sizes:
• 20 bp – 55%
• 40 bp – 68%
• 120 bp – 76%
• 200 bp – 80%
• 8 – 9 kbp – 75%
• >10 kbp – < 60%
If lower