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Jilite Wang et al.
Bioscience, biotechnology, and biochemistry, 79(3), 456-461 (2014-11-07)
Dietary plant protein is well known to reduce serum cholesterol levels. Rice bran is a by-product of rice milling and is a good source of protein. The present study examined whether feeding rats a high-cholesterol diet containing 10% rice bran
Martin Gustavsson et al.
Methods in enzymology, 570, 233-260 (2016-02-29)
The development of methods for expression and purification of seven-transmembrane receptors has led to an increase in structural and biophysical data and greatly improved the understanding of receptor structure and function. For chemokine receptors, this has been highlighted by the
Paola Mura et al.
International journal of pharmaceutics, 475(1-2), 306-314 (2014-09-10)
Transdermal administration of clonazepam, a poorly water-soluble benzodiazepine, is an interesting strategy for overcoming the drawbacks of its oral administration. With this aim, two nano-carrier formulations, based on ultra-deformable liposomes and microemulsions, have been developed to favour clonazepam transdermal delivery.
Nicholas A Swanson et al.
STAR protocols, 2(4), 100960-100960 (2021-11-27)
Bacteriophages of the Podoviridae family densely package their genomes into precursor capsids alongside internal virion proteins called ejection proteins. In phage T7 these proteins (gp14, gp15, and gp16) are ejected into the host envelope forming a DNA-ejectosome for genome delivery.
Suguru Yamasaki et al.
Scientific reports, 9(1), 177-177 (2019-01-19)
During infection, Salmonella senses and responds to harsh environments within the host. Persistence in a bile-rich environment is important for Salmonella to infect the small intestine or gallbladder and the multidrug efflux system AcrAB-TolC is required for bile resistance. The
Mavis Jiarong Li et al.
Current protocols in protein science, 98(1), e100-e100 (2019-11-21)
Lipid nanodiscs provide a native-like lipid environment for membrane proteins, and they have become a valuable platform for the study of membrane biophysics. A range of biophysical and biochemical analyses are enabled when membrane proteins are captured in lipid nanodiscs.
Yuri Kashima et al.
PloS one, 9(8), e105073-e105073 (2014-08-22)
Royal jelly (RJ) intake lowers serum cholesterol levels in animals and humans, but the active component in RJ that lowers serum cholesterol level and its molecular mechanism are unclear. In this study, we set out to identify the bile acid-binding
Volkan K Köseoğlu et al.
Infection and immunity, 87(7) (2019-04-17)
Shigella flexneri is an intracellular bacterial pathogen that invades epithelial cells in the colonic mucosa, leading to bloody diarrhea. A previous study showed that S. flexneri forms biofilms in the presence of bile salts, through an unknown mechanism. Here, we
Infiltration of pressurized promoter solutions in a mesoporous silica
Punyamurtula and Qiao
Microporous Mater., 103, 5-5 (2007)
Mayu Kakehi et al.
Toxicological sciences : an official journal of the Society of Toxicology, 147(2), 360-369 (2015-07-17)
There are various interspecies differences in xenobiotic-metabolizing enzymes. It is known that cats show slow glucuronidation of drugs such as acetaminophen and strong side effects due to the UGT1A6 pseudogene. Recently, the UGT1A6 pseudogene was found in the Northern elephant
Thuc Hue Ly et al.
Nature communications, 7, 10426-10426 (2016-01-28)
Grain boundaries in monolayer transition metal dichalcogenides have unique atomic defect structures and band dispersion relations that depend on the inter-domain misorientation angle. Here, we explore misorientation angle-dependent electrical transport at grain boundaries in monolayer MoS2 by correlating the atomic
Lado Otrin et al.
Nature communications, 12(1), 4972-4972 (2021-08-19)
A variety of artificial cells springs from the functionalization of liposomes with proteins. However, these models suffer from low durability without repair and replenishment mechanisms, which can be partly addressed by replacing the lipids with polymers. Yet natural membranes are
Pedro F Esteban et al.
Journal of neuroscience methods, 337, 108680-108680 (2020-03-08)
Cannabinoid receptor 1 (CB1) identification by western blot (WB) has generated a great deal of controversial data making the interpretation of the results difficult. Our purpose is to find the most adequate experimental conditions to detect CB1 by WB and
F Peter Guengerich et al.
Nature protocols, 4(9), 1245-1251 (2009-08-08)
Cytochrome P450 (P450) enzymes are important in the metabolism of steroids, vitamins, carcinogens, drugs and other compounds. Two of the commonly used assays in this field are the measurements of total P450 and NADPH-P450 reductase in biological preparations. A detailed
Peter T McKenney et al.
Cell host & microbe, 25(5), 695-705 (2019-04-30)
Vancomycin-resistant Enterococcus (VRE) are highly antibiotic-resistant and readily transmissible pathogens that cause severe infections in hospitalized patients. We discovered that lithocholic acid (LCA), a secondary bile acid prevalent in the cecum and colon of mice and humans, impairs separation of growing VRE diplococci
Fang Fang et al.
Journal of bacteriology, 191(18), 5743-5757 (2009-07-14)
Commensal lactobacilli frequently produce bile salt hydrolase (Bsh) enzymes whose roles in intestinal survival are unclear. Twenty-six Lactobacillus salivarius strains from different sources all harbored a bsh1 allele on their respective megaplasmids. This allele was related to the plasmid-borne bsh1
Cynthia L Morris-Kukoski et al.
Journal of analytical toxicology, 38(9), 628-636 (2014-08-08)
Samples from a self-proclaimed cocaine (COC) user, from 19 drug users (postmortem) and from 27 drug chemists were extensively washed and analyzed for COC, benzoylecgonine, norcocaine (NC), cocaethylene (CE) and aryl hydroxycocaines by liquid chromatography-tandem mass spectrometry. Published wash criteria
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