Advanced gene editing

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facet applications:Advanced gene editing

facet applications:DNA and RNA purification

facet applications:Nucleic acid gel electrophoresis

facet applications:PCR

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The purpose of Hot Start PCR is to inhibit the PCR reaction in order to reduce nonspecific amplification, prevent the formation of primer dimers, and increase product yields.

Selecting DNA, RNA, and PCR Fragment Markers and Ladders for Gel Electrophoresis

Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.

PCR Assay Optimization and Validation

PCR assay guide navigates you through primer validation and other assay optimization factors to ensure high sensitivity and specificity for optimum DNA/ RNA quantification.

Colony PCR

Colony PCR reagents and our colony PCR protocol using REDExtract-N-Amp™ PCR ReadyMix™ and JumpStart™ REDTaq® PCR ReadyMix™ reagents.

DNA / Protein Electrophoresis and Troubleshooting Tables

A highly efficient method to concentrate DNA for forensic STR genotyping using DNAstable®

Forensic laboratories routinely use STR genotyping for identity testing of biological samples. However, forensic samples often contain low copy numbers of target DNA, making it difficult to obtain complete STR profiles.

5'/3' RACE Kit, 2nd Generation Troubleshooting

In addition to the troubleshooting provided in the product manual, most probably the efficiency of the tailing reaction performed by Terminal Transferase could be impaired. This could occur due to several reasons (which will not only affect the control reaction

Restorase® DNA Polymerase

Restorase® was developed for researchers unable to achieve amplification of damaged DNA templates when using other commercially available DNA polymerases.

Polymerase Chain Reaction (PCR) Basics

Polymerase chain reaction (PCR) is a technique that results in exponential amplification of a target DNA sequence.

ECACC Nucleic Acids

DNA, RNA, cDNA derived from ECACC mammalian cell lines allow screening for genes or expression patterns to identify lines most suitable for specific research.

Long and Accurate PCR

Long and accurate PCR applications address the needs for longer read lengths, greater fidelity and higher yields than that which can be achieved with Taq DNA polymerase.

Downstream Applications after Genomic DNA Purification

This page shows downstream applications after genomic DNA purification.

Sodium acetate buffer solution for molecular biology

Sodium Acetate Buffer provider. 5+ decades providing high-quality buffer products that maintain consistent performance for molecular biology .

Plasmid DNA Preparation Troubleshooting

This page shows possible causes and solutions for problems that may occur during plasmid DNA preparation.

RNA Preparation Troubleshooting

This page shows possible causes and solutions for problems that may occur during RNA preparation.

Oligonucleotide Quality Control by Analytical HPLC

In many cases oligonucleotides require quantitative analysis to verify specifications have been met. MALDI-TOF and ESI-MS can provide qualitative-quantification for purity.

Complete Solutions for PCR Assay Development

Fit-for-use products offer the quality, consistency & documentation necessary for every step of your IVD development and manufacturing process.

Successful Transduction Using Lentivirus

Get tips for handling lentiviruses, optimizing experiment setup, titering lentivirus particles, and selecting helpful products for transduction.

Dual-Labeled Probes in Digital PCR

Learn more about how digital PCR (dPCR) is used for absolute quantification and for analysis of minority sequences.

Working with PCR

General Considerations; Factors to Consider in RT-PCR; Prevention of Carryover Contamination; Troubleshooting

KAPA Long Range PCR Kits FAQs

Review some of the most asked questions regarding the KAPA Long Range PCR system, which is a blend of Taq DNA polymerase and an engineered archaeal DNA polymerase with proofreading capability.

KAPA3G Plant PCR Kits FAQs

Amplify up to 5 kb fragments from purified plant DNA extracted using commercial kits or CTAB-based methods. Review some of the most asked questions regarding direct PCR from leaf disks, seeds and other plant tissues.

GC-RICH PCR System Troubleshooting

GC-RICH Amplification of Polymerase Chain Reaction (PCR) System Troubleshooting.

Long-term Storage of Blood in DNAgard® Blood

Two separate studies were conducted to analyze long-term storage of blood in DNAgard Blood stabilization reagent.

Extract-N-Amp™ Blood PCR Kit Protocol

The Extract-N-Amp Blood PCR Kits contain all the reagents needed to rapidly extract and amplify human genomic DNA from whole blood, whole blood dried on a blood card, and cultured mammalian cells.

PCR Master Mix

PCR master mix simplifies PCR/RT-PCR with components like DNA polymerase, dNTPs, MgCl2, and buffer, available commercially or DIY.

PCR/qPCR/dPCR Assay Design

PCR workflow's variability influenced by sample source, reverse transcription, and assay design, impacting reproducibility.

Proteinase K: Introduction & Applications

Proteinase K aids in molecular biology applications by digesting structural proteins, removing nucleases, and isolating intact genomic DNA.

FastStart™ Taq DNA Polymerase Protocol & Troubleshooting

PCR success relies on enzyme, buffer choice, template purity, primer concentration, and nucleotide quality.

Validating CRISPR/Cas9-mediated Gene Editing

Validate CRISPR gene editing experiments easily with Sigma-Aldrich® T7E1 mismatch detection kit, ensuring successful editing.

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