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Water for PCR Techniques
Learn how water impurities can affect PCR and related methodologies. High purity water purified using ultrafiltration is suitable for PCR and related techniques.
Long Oligos
Techniques for long oligo synthesis and purification offer superior quality unmatched by other suppliers.
Methods for Enhancing Lentiviral Transduction Efficiency
Methods for lentiviral transduction of Jurkat cells were compared. Spinoculation was compared with overnight incubation with polybrene (hexadimethrine bromide) and fibronection-coated plates.
Water for Nuclease-sensitive Molecular Biology Studies
Nuclease-free water (or PCR water) is needed in molecular biology to avoid sample degradation during PCR, sequencing, etc. Ultrafiltration provides a quicker, safer, and more sustainable alternative to DEPC treatment when preparing RNase-free and DNase-free water.
Water for pH Measurement and Buffer Preparation
Learn how water impurities can affect pH. Water quality defined as pure water or Type 2 water is recommended to prepare buffer solutions or measure pH.
CRISPR-based Gene Activation
Sigma has developed a gene activation system based on a fusion of dCas9 to the catalytic histone acetyltransferase (HAT) core domain of the human E1A-associated protein p300.
Lentivirus Cell Line
Using lentivirus as a means to deliver shRNAs has become standard practice in many labs exploring RNAi.
TRC Design and pLKO.1 Vector FAQs
MISSION® shRNA clones in pLKO.1-Puro vector allow bacterial culture propagation and mammalian cell selection for gene silencing studies.
Reverse Transfection of Plasmid DNA
Automation is used for many applications to reduce variation caused by manual handling and to obtain reproducible results in high-throughput assays. High-throughput applications, such as knockdown studies or target screenings, often include cell transfection.
Safe Lentivirus Handling
Lentiviral vector systems prioritize safety features, with design precautions preventing replication. Good handling practices are essential for use.
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