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Ascentis® Express C8 U/HPLC Columns
When a C18 doesn′t give the desired separation, or your sample contains compounds that are known to be difficult to retain or resolve on a C18, consider changing to an Ascentis® Express C8 column.
High Resolution and High Efficiency Separations of mAbs and ADCs Using Proteomix HIC Columns
Hydrophobic interaction chromatography, a protein separation technique, has been commonly used as an orthogonal method to SEC and ion exchange for the characterization of mAbs.
UHPLC Analysis of Conjugated Estrogens on Ascentis ® Express C18
Separation of Estriol 3-(β-D-glucuronide) sodium salt; β-Estradiol 3-(β-D-glucuronide) 17-sulfate dipotassium salt; Estriol 3-sulfate sodium salt; β-Estradiol 3,17-disulfate dipotassium salt, ≥95%; β-Estradiol 17-(β-D-glucuronide) sodium salt; β-Estradiol 3-(β-D-glucuronide) sodium salt; Estrone 3-(β-D-glucuronide) sodium salt; β-Estradiol 3-sulfate sodium salt, ≥93%; Estriol, ≥97%; Estrone...
HPLC Analysis of Proteins on TSKgel® BioAssist S
Separation of Ribonuclease A from bovine pancreas, Type I-A, powder, ≥60% RNase A basis (SDS-PAGE), ≥50 Kunitz units/mg protein; α-Chymotrypsinogen A from bovine pancreas, essentially salt-free, lyophilized powder; Cytochrome c from bovine heart, ≥95% based on Mol. Wt. 12,327 basis;...
HPLC Analysis of Pain Relievers on Ascentis® Express C18
Separation of Acetaminophen, meets USP testing specifications, 98.0-102.0%, powder; Caffeine, meets USP testing specifications, anhydrous; Benzoic acid, meets USP testing specifications; Aspirin, meets USP testing specifications
A Quantitative Mass Spectrometry-based Approach for Identifying Protein Kinase Clients and Quantifying Kinase Activity
A quantitative mass spectrometry-based approach for identifying protein kinase clients and quantifying kinase activity
HPLC Analysis of Sugars by HILIC Chromatography using Ascentis® Express OH-5 Column with ELSD Detection
Separation of Dextrose, Pharmaceutical Secondary Standard; Certified Reference Material; Sucrose; Maltitol, Pharmaceutical Secondary Standard; Certified Reference Material
HPLC Analysis of Polyglutamate Metabolites of Methotrexate on TSKgel® NH2-100 and ODS-100V
Separation of Methotrexate (MTX)-PG; Methotrexate (MTX)-PG2; Methotrexate (MTX)-PG3 ; Methotrexate (MTX)-PG4; Methotrexate (MTX)-PG5; Methotrexate (MTX)-PG6; Methotrexate (MTX)-PG7
HPLC Analysis of Cisplatin and Monohydrated Cisplatin on SeQuant ZIC-HILIC
Antineoplastic agents separated with excellent resolution.
HPLC Analysis of Tricyclic Antidepressants on Ascentis® Express 2.7 μm RP-Amide
-isomer basis, ≥98% (GC), 15% Z-isomer basis, powder; Norclomipramine hydrochloride, ~99% (TLC), powder; Imipramine hydrochloride solution, drug standard, 1.0 mg/mL in methanol; Amitriptyline hydrochloride, ≥98% (TLC), powder; Clomipramine hydrochloride, ≥98% (HPLC), powder
LC/MS Analysis of Antiarrhythmic Drugs and Metabolites on Ascentis® Express HILIC
The benefit of HILIC over traditional reversed-phase chromatography is two-fold for both sample introduction and analyte detection. First, the high acetonitrile concentration of HILIC mobile phases allows for direct analysis of precipitated plasma samples without the need for additional sample...
HPLC Analysis of Xanthines on Purospher® STAR RP-18 endcapped
Xanthine is a purine base found in most human body tissues and fluids as well as in other organisms. Methylated xanthines (methylxanthines), which include caffeine, paraxanthine, theobromine, and theophylline, commonly used for their effects as mild stiµlants and as bronchodilators...
HPLC Analysis of Sugars on Ascentis® Si
Separation of Sucrose; Maltose; Maltotriose
Ascentis® Express OH5 U/HPLC Columns
High-speed Ascentis® Express OH5 HPLC columns are designed for enhanced HILIC partitioning and limited ion exchange retention
LC/MS/MS Analysis of Vitamin D Metabolites on Ascentis® Express F5
This application shows an Ascentis Express F5 provided rapid resolution of the isobaric vitamin D metabolites. MS detection provides the necessary secondary resolution.
HPLC Analysis of Protein Standards (1) on BIOshell™ A400 Protein C4
For use as a marker in SDS-PAGE; Albumin from chicken egg white, For use as a marker in SDS-PAGE; L-Lactic Dehydrogenase from rabbit muscle, Type XI, lyophilized powder, 600-1,200 units/mg protein
Physical Stability of a Gel Filtration Column for Antibody Analysis
The results of this column stability study show that TSKgel G3000SWXL are highly stable; the efficiency and peak symmetry values show minimal variation from the first to the 1,000th injection.
Dyestuff Analyses on Historic Objects by LC-ESI-MS/MS Using a Superspher RP-18 HPLC Column
In this study, LC-ESI-MS/MS under reversed phase conditions using a Superspher RP-18 column is applied to analyze dyestuffs of a reddish historic textile from South America.
An LC/MS Peptide Standard for Rapid System Suitability Assessment and Retention Time Translation Across LC/MS Platforms
Standards are critical in liquid chromatography/mass spectrometry (LC/MS) based proteomics to ensure optimal and consistent system performance before, during, and after sample analysis.
Oligonucleotide Quality Control by Analytical HPLC
In many cases oligonucleotides require quantitative analysis to verify specifications have been met. MALDI-TOF and ESI-MS can provide qualitative-quantification for purity.
Ascentis ® Express HILIC U/HPLC Columns
For separation of polar compounds including polar neutrals, polar acids, and polar and non-polar basic amines use our Ascentis® Express HILIC column.
LC/MS/MS Analysis of Testosterone and Metabolites on Ascentis® Express C18
Separation of Testosterone solution, 1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material; 17α-Methyltestosterone solution, 1.0 mg/mL in 1,2-dimethoxyethane, ampule of 1 mL, certified reference material; 5α-Dihydrotestosterone (DHT) solution, 1.0 mg/mL in methanol, ampule of 1 mL, certified...
HPLC Analysis of Z-Drugs on Ascentis® Express ES-Cyano 2.0 μm
An rapid method for the siµLtaneous determination of the Z-drugs or sleep aids: zopiclone, zolpidem, and zaleplon is presented here. The need for greater analytical capacity and throughput for the analysis of sleep aid medicines (Z-drugs) in forensic toxicology laboratories...
Supelco Preparative HPLC products for Pharmaceutical Development and Production
Supelco Preparative HPLC products for Pharmaceutical Development and Production
Validation of RNAi Knockdown Using Multiple Reaction Monitoring and Protein-AQUA
This discussion will highlight some of these methodologies, namely, the use of Multiple Reaction Monitoring (MRM) and Protein-AQUA.
Peptide Sample Amount Determination
Determining the amount of desired product in a peptide sample.
Determination of Sucralose in Soy Sauce using HPLC-ELSD following the Chinese National Standard (GB) Method
The most frequently used synthetic sweeteners are: saccharin, cyclamate, aspartame, and sucralose (E955), and this application illustrate the analysis of sucralose from soy sauce following the current Chinese national standard method.
Ascentis® Express F5 (pentafluorophenyl) U/HPLC Columns
Enhanced selectivity for stereoisomers and closely related compounds with alternate selectivity from C18 phases for basic, acidic, or neutral compounds
Ascentis® Express RP-Amide Columns for HPLC and UHPLC
Improve your separation of basic compounds with our Ascentis® Express RP-Amide HPLC columns from the Supelco® portfolio.
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