Mammalian cell culture

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Selection of the right fusion partner cell line is critical to obtain desired attributes of the hybridoma. Explore hybridoma fusion partners cell lines.

3D Neurosphere Culture Media

3D neurosphere culture protocol to expand human U-87 MG glioblastoma cells in serum-free/xeno-free stem cell culture media. Explore over 350 PromoCell products on Sigma.com.

RPMI-1640 Media

RPMI-1640 is used for the culture of human normal and neoplastic leukocytes, when properly supplemented, has demonstrated wide applicability for supporting growth of many types of cultured cells, including fresh human lymphocytes.

Common Cell Culture Problems: Precipitates

Common Cell Culture Problems: Precipitates -- When contamination is ruled out, turbidity in cell culture media is often explained by the precipitation of metals, proteins, and other media components. Precipitates can be harmful to cell health, as they may alter

Lipid-induced Insulin Resistance

Lipid Induced Insulin Resistance

Platelet Derived Growth Factors (PDGF)

Platelet-Derived Growth Factor is the principal mitogen found in mammalian serum and is released from platelets during clot formation.

Applications of Percoll in Other Cell Types

The table was compiled to assist the researcher in selecting references most likely to contain relevant information regarding use of Percoll in various cell types.

Lipoic (Thioctic) Acid, in Cell Culture

How lipoic acid (thioctic acid) and other cell culture components affect the performance of serum-free, protein-free cell culture systems used for biomanufacturing heterologous proteins including monoclonal antibodies.

Removal of Percoll after Centrifugation

This page shows methods to remove Percoll from a sample after centrifugation.

Cell Culture Protocol 5: Subculture of Suspension Cell Lines

Cell culture protocol for passaging and splitting suspension cell lines

Autophagy in Cancer Promotes Therapeutic Resistance

We presents an article on Autophagy in Cancer Promotes Therapeutic Resistance

Hematopoietic Cytokines

Read article on hematopoietic cytokines and hematopoiesis

Fibroblast Growth Factor Family (FGF)

Fibroblast growth factors in cell culture and various growth factors for your research

Cell Death Troubleshooting in Cell Culture

Here we discuss causes of cell death, and how to assess and prevent these causes.

Cell Culture Protocol 10: Testing Cells for Mycoplasma Contamination by Hoechst DNA Staining

Cell culture protocol for testing cell lines for mycoplasma contamination using indirect DNA staining using Hoechst 33342.

Human Hematopoietic Progenitor Cells (HPC) and Growth Media

CD34+ Progenitor Cells are suitable for diverse studies, as well as for the directed differentiation into more committed blood cell phenotypes and endothelial lineages.

Media Formulations

Compare all of the formulation tables for cell culture in one place.

Dulbecco's Modified Eagle's Medium (DMEM) Formulation

L-Ornithine in Cell Culture

Discover L-ornithine, an important amino acid supplement, explore how L-ornithine is used in CHO and iPSC culture, and discover how poly-L-ornithine is used to enhance cell attachment.

DMEM/nutrient Mixture F-12 Formulation

Dulbecco's Modified Eagle's Medium/Ham's Nutrient Mixture F-12 (DME/F12) Formulation

Ascorbate in Cell Culture

Importance and uses of ascorbate in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell, cultures

Biotin in Cell Culture

Importance and uses of biotin in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell, cultures

MEM (Minimum Essential Medium Eagle)

A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.

Selenium in Cell Culture

Understand the role of selenium in cell culture media formulations. The Media Expert library explains how culture media ingredients support cell growth.

Human Serum Albumin (HSA)

Learn more about the cell culture supplements human serum albumin (HSA) and recombinant human serum albumin (rHSA). Explore their applications in cell culture and beyond.

Cell Culture Fundamentals: Safety Aspects of Cell Culture

Overview on how to maintain proper cell culture laboratory safety including steps on performing lab risk assessments, surface disinfection, biohazard and waste removal. Free ECACC handbook download.

Good Cell Banking Practices

Overview of good cell banking practices for cell line cryopreservation purposes.

Calcium in Cell Culture

Calcium is an ionically stable divalent cation with important beneficial and toxic properties in cell culture. It is a component of a wide range of cell culture media.

Quality Control Considerations in Cell Culture

Quality control guidelines to maintain high quality authenticated and contamination-free cell cultures. Free ECACC handbook download.

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