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Protein labeling and modification

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Coupling to Microparticles
Biochemicals Coupling to Microparticles. Besides Proteins, DNA, lectins and many other species have been coupled to microparticles.
Purification or Removal of Biotin and Biotinylated Biomolecules with Magnetic Beads
This page shows how to Purify or remove biotin and biotinylated biomolecules with magnetic beads.
Coupling Protocol for Primary Amine of a Ligand
This page shows coupling through the primary amine of a ligand with a NHS-activated Sepharose High Performance, NHS-activated Sepharose 4 Fast Flow and CNBr-activated Sepharose from Cytiva.
Removal of GST Tag by Enzymatic Cleavage
This page how to remove GST tags by enzymatic cleavage using Cytiva products.
Neuraminidase (Sialidase) Protocol
Neuraminidase can be used to cleave sialic acids from proteins. In this protocol, the enzyme from Vibrio cholerae is used on fixed cells.
Mass Spectrometry of Glycans
Explore mass spectrometry analysis of glycans for glycomic & glycoproteomic neutral & acidic glycan analysis. See a general mass spec glycan analysis procedure.
Clean-up of Antibody Labeling Reactions Using Amicon Ultra Filters
Using a standard Amicon® Ultra centrifugal filter, a few rounds of diafiltration can efficiently remove unincorporated label from an antibody labeling reaction without diluting the sample.
Tag Removal by Enzymatic Cleavage
This protocol shows how to remove histidine tags by enzymatic cleavage using Cytiva products.
Conjugating Alkaline Phosphatase to Antibodies and Other Proteins
Protocol for the one-step, glutaraldehyde coupling method to conjugation alkaline phosphatase to antibodies and other proteins.
Peptide Quick Tips
Peptide Quick Tips. Helpful guidelines for proper storage and resuspension of your peptide for optimal performance in your assay.
Purification or Removal of Biotin and Biotinylated Substances
This page shows how to purify or remove biotin and biotinylated substances with Streptavidin Sepharose High Performance from Cytiva.
N-Glycosidase F Protocol & Troubleshooting
N-Glycosidase F Protocol & Troubleshooting
Enzymatic Deglycosylation
Enzymatic Deglycosylation Strategies: Sequential hydrolysis of individual monosaccharides from glycans can be useful for the elucidation of the structure and function of the glycan component.
Calf Intestinal (CIP) Alkaline Phosphatase for Nucleic Acid Dephosphorylation
CIP is used to remove 5’-phosphate groups from DNA, RNA and both ribo and deoxy-ribonucleoside triphosphates. Detailed protocol on how to dephosphorylate DNA.
Alkaline Phosphatase Protocol
Alkaline phosphatase (AP) is a non-specific phosphomonoester hydrolase that catalyzes the hydrolysis of a wide variety of organic monophosphates.
Exosome Labeling Protocol with PKH Lipophilic Dyes
PKH dyes label exosomes for tracking experiments in vitro and in vivo, detailed protocol provided.