Merck
Search Within
Applications
Content Type
Applied Filters:
Applications:Transfection and gene editing
Content Type:Technical Article
Showing 1-21 of 21 results
Sort by Relevance
Successful Transduction Using Lentivirus
Get tips for handling lentiviruses, optimizing experiment setup, titering lentivirus particles, and selecting helpful products for transduction.
Development of a New Advanced KSOM Mouse Embryo Media that Enables Dual-Use Embryo Culture and Cell Handling Applications
Advanced KSOM mouse embryo media that can be used as a single medium for both harvest and culture of mouse embryos to facilitate creation of transgenic knockout mice.
Lentiviral Transduction
In this exercise we will be demonstrating lentiviral transduction on two different cell lines.
Detect, Visualize and Quantify Single Post-Translational Modifications
Sigma-Aldrich presents a Biofiles on Detect, Visualize and Quantify Single Post-Translational Modifications
Functionalized PEI and Its Role in Gene Therapy
Gene therapy has become one of the most discussed techniques in biomedical research in recent years.
MVM Resistance Through Genetic Engineering
As part of the CHOZN brand promise to enable the development of better biologics and safer manufacturing processes, we have identified a gene target (SLC35A1) that upon elimination of expression by gene editing, results in a CHO cell line that
A Versatile new Lipofection Reagent for co-transfection of DNA and RNA: X-tremeGENE™ siRNA Transfection Reagent
Small inhibitory RNAs (siRNAs) have become the focus of interest in many laboratories. For the first time, these molecules offer an easy way to knock down the expression of selected genes in mammalian cells without having to resort to classical
Validation of RNAi Knockdown Using Multiple Reaction Monitoring and Protein-AQUA
This discussion will highlight some of these methodologies, namely, the use of Multiple Reaction Monitoring (MRM) and Protein-AQUA.
Pooled Lentiviral shRNA Screens to Identify Essential Cancer Pathways
Presenting an article on Pooled Lentiviral shRNA Screens to Identify Essential Cancer Pathways
Introduction to Cell Transfection
Transfection is the introduction of DNA, RNA, or proteins into eukaryotic cells and is used in research to study and modulate gene expression. Thus, transfection techniques and protocols serve as an analytical tool that facilitates the characterization of genetic functions
Whole Genome Amplification for Single Cell Biology
Whole genome amplification (WGA) offers a means to overcome the above restrictions for single-cell genomic analyses. WGA has been described as a non-specific amplification technique that affords an amplified product completely representative of the initial starting material.
Methods for Enhancing Lentiviral Transduction Efficiency
An experiment to directly compare three methods of lentiviral transduction of Jurkat cells was conducted in order to determine the method that yields the greatest transduction efficiency.
CRISPR Engineered MDCKII Cells Without Canine P-glycoprotein
Madin-Darby Canine Kidney (MDCK) cells are one of the most widely used cell lines for a variety of research applications, including drug permeability and transporter studies.
Reverse Transfection of Plasmid DNA
Automation is used for many applications to reduce variation caused by manual handling and to obtain reproducible results in high-throughput assays. High-throughput applications, such as knockdown studies or target screenings, often include cell transfection.
siRNA Delivery in Mammalian Cell Lines
siRNAs are effectively delivered into mammalian cell lines that have been difficult or even impossible to transfect with siRNA when using other commercially available lipofection reagents.
Lentiviral Packaging
The goal of this exercise is to learn lentiviral packaging. For this example we will be using the HEK 293T cell line.
Tips for Cell Engineering Using Cas9-GFP CRISPR Plasmids
CRISPR endonucleases have shown wide variation in their activity, even among multiple CRISPRs designed within close genomic proximity.
siRNA Design, Manufacturing, Transfection & Detection
RNA interference (RNAi) is a natural biological mechanism wherein short inhibitory RNA (siRNA) duplexes induce potent inhibition of gene expression.
Antibiotic Kill Curve
Antibiotic kill curve is a dose response experiment in which mammalian cells are subjected to increasing amounts of selection antibiotic
Why Use Antibiotics in Cell Culture?
Know when to use antibiotics to prevent bacterial or fungal, mycoplasma, or viral contamination in cell culture and find suitable antibiotics or other biological agents.
Troubleshooting for Molecular Cloning
Molecular cloning is the process of inserting the gene-of-interest (GOI) into a plasmid vector and this vector is then inserted into a cell that expresses the protein encoded by the GOI. Once protein is expressed in the cell, the protein