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CHOZN® Cell Line Engineering & Development

Generating and isolating high-producing cell lines or clones can be costly and labor-intensive. Instead, free your R&D to focus on developing new therapeutics by outsourcing the engineering of production cell lines to our CHOZN® Platform Development Team of highly trained scientists.

We can support your biomanufacturing and research with years of expertise in genome editing for Chinese hamster ovary (CHO) cell lines. Our customized services use a variety of molecular biology methods, including our own CompoZr® zinc finger nuclease (ZFN) technology, to deliver efficient cell lines that help reduce product costs and timelines.

Services include:

  • Engineering CHO cell lines for bioproduction
  • Custom development in your CHO cell line
  • Custom development of recombinant cell lines within our own CHOZN® GS CHO cell line

Each cell line engineering project is divided into phases, invoiced only after a phase has been completed. We stay in close technical communication with your R&D team so that projects progress smoothly, advancing to each new phase when there is mutual agreement.

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Outsourcing process for CHO cell line engineering and genetic modification

Project Phases for Standard Cell Line Engineering

  • Stage 1: Program initiation and technology transfer
  • Stage 2: ZFN design and validation
  • Stage 3: ZFN preparation, transfection, and activity confirmation
  • Stage 4: Single-cell cloning and characterization
  • Stage 5: Mycoplasma and sterility testing and clone delivery

Project Phases for Standard Cell Line Development

  • Stage 1: Program initiation and technology transfer
  • Stage 2: Expression vector construction
  • Stage 3: Stable pool generation and characterization
  • Stage 4: Single-cell cloning and characterization
  • Stage 5: Genetic stability study (optional)
  • Stage 6: Protein quality assessment (optional)
  • Stage 7: Clone safety testing and transfer
  • Stage 8: Custom media, feed, and process development (optional)
  • Stage 9: MCB generation and characterization (optional)

To discuss your needs, simply contact our team and a scientist will get in touch. We will then prepare a proposal detailing your project, which can begin with your approval.

Genetic Modification with CompoZr® Zinc Finger Nucleases (ZFNs)

A powerful tool for creating novel CHO lines, ZFNs are a class of engineered DNA-binding proteins that create double-strand breaks in DNA at user-specified locations. These double-strand breaks stimulate the cell's natural DNA-repair processes via homologous recombination (HR) and non-homologous end joining (NHEJ). By applying well-established and robust protocols, our team can make targeted genome edits such as:

  • Cell lines with targeted gene deletions (knockouts)
  • Cell lines with targeted integrations (additions/knock-ins)
  • Cell line modifications

We offer ZFN reagents in DNA plasmid format, as well as mRNA. Still another option is fluorescently tagged ZFNs, enabling fluorescence-activated cell sorting (FACS) that can result in an enriched pool of desired cells.

The Perfect Fit for your Process

  • Expertise in biopharmaceutical development projects
  • Modular service options customized to your precise needs
  • Cost-efficient access to the latest technologies (CompoZr® ZFNs, MISSION® RNAi)
  • Effective CHO cell lines delivered for immediate use
  • Assured documentation and quality control
  • Dedicated management of timelines and deliverables

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