Merck

Specific cleavage of tRNA by nuclease S1.

Nucleic acids research (1975-06-01)
F Harada, J E Dahlberg
ABSTRACT

Nuclease S1 specifically hydrolizes tRNAs in their anticodon loops, forming new 5' phosphate and 3' OH ends. Some single-stranded regions are not cut by nuclease S1. The strong preference of nuclease S1 for the anticodon region can be used for rapid identification of an anticodon-containing oligonucleotide and subsequent identification of the probable amino acid specificity of tRNA.

MATERIALS
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Product Description

Sigma-Aldrich
Nuclease S1 from Aspergillus oryzae, for single-strand DNA/RNA digestion