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  • Sea snake cathelicidin (Hc-cath) exerts a protective effect in mouse models of lung inflammation and infection.

Sea snake cathelicidin (Hc-cath) exerts a protective effect in mouse models of lung inflammation and infection.

Scientific reports (2019-04-17)
Simon R Carlile, Jenna Shiels, Lauren Kerrigan, Rebecca Delaney, Julianne Megaw, Brendan F Gilmore, Sinéad Weldon, John P Dalton, Clifford C Taggart
ABSTRACT

We investigated the anti-inflammatory and antibacterial activities of Hc-cath, a cathelicidin peptide derived from the venom of the sea snake, Hydrophis cyanocyntus, using in vivo models of inflammation and infection. Hc-cath function was evaluated in in vitro, in vivo in the wax moth, Galleria mellonella, and in mouse models of intraperitoneal and respiratory Pseudomonas aeruginosa infection. Hc-Cath downregulated LPS-induced pro-inflammatory responses in macrophages and significantly improved the survival of P. aeruginosa infected G. mellonella over a 5-day period. We also demonstrated, for the first time, that Hc-cath can modulate inflammation in a mouse model of LPS-induced lung inflammation by significantly reducing the release of the pro-inflammatory cytokine and neutrophil chemoattractant, KC, resulting in reduced cellular infiltration into the lungs. Moreover, Hc-cath treatment significantly reduced the bacterial load and inflammation in mouse models of P. aeruginosa intraperitoneal and respiratory infection. The effect of Hc-cath in our studies highlights the potential to develop this peptide as a candidate for therapeutic development.

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Supelco
Vial, Chromacol, PTFE (non-disposable sleeve), O.D. × H 12 mm × 32 mm, for use with Supelco part numbers 27312, 27313, 27314, 27315, pkg of 40 ea