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Silicatein-mediated incorporation of titanium into spicules from the demosponge Suberites domuncula.

Cell and tissue research (2009-12-17)
Filipe Natalio, Enrico Mugnaioli, Matthias Wiens, Xiaohong Wang, Heinz C Schröder, Muhammad Nawaz Tahir, Wolfgang Tremel, Ute Kolb, Werner E G Müller
ABSTRACT

Primmorphs (a three-dimensional sponge primary cell culture system) have been revealed to be a cell/tissue nano-factory for the production of tailor-made hybrid nanostructures. Growth of primmorphs is stimulated by the presence of a titanium alkoxide precursor tolerating titania (TiO2) concentrations up to 250 microM. The presence and activity of silicatein in primmorphs has been analyzed by gel electrophoresis and Western blotting. Results of studies by scanning transmission electron microscopy with energy-dispersive X-ray spectroscopy have revealed silica and titania to be co-localized on nanosized spicules. Our findings suggest that the incorporation of titania into the nanosized spicule is enzymatically mediated via active silicatein in an orchestrated mechanism.

MATERIALS
Product Number
Brand
Product Description

Supelco
SYPRO® Ruby Protein Gel Stain
Sigma-Aldrich
Anti-Rabbit IgG (whole molecule)–Alkaline Phosphatase antibody produced in goat, affinity isolated antibody, buffered aqueous glycerol solution