Preventive effect of phytic acid on lysosomal hydrolases in normal and isoproterenol-induced myocardial infarction in Wistar rats.

Preventive effect of phytic acid on lysosomal hydrolases in normal and isoproterenol-induced myocardial infarction in Wistar rats.

Toxicology mechanisms and methods (2015-01-07)

E Brindha, M Rajasekapandiyan

ABSTRACT

This study was aimed to evaluate the preventive role of phytic acid on lysosomal enzymes in isoproterenol (ISO)-induced myocardial infarction (MI) in male Wistar rats. Rats subcutaneously injected with ISO (85 mg/kg) at an interval of 24 h for two days showed a significant increase in the activities of lysosomal enzymes (glucuronidase, N-acetyl glucosaminidase, galactosidase, cathepsin-B and cathepsin-D) were increased significantly in serum and the heart of ISO-induced rats, but the activities of glucuronidase and cathepsin-D were decreased significantly in the lysosomal fraction of the heart. Pretreatment with phytic acid (25 and 50 mg/kg) daily for a period of 56 d positively altered activities of lysosomal hydrolases in ISO-induced rats. Thus, phytic acid possesses a cardioprotective effect in ISO-induced MI in rats.

MATERIALS

Product Number

Brand

Product Description

705551

Sigma-Aldrich

Hydrogen chloride solution, 3 M in cyclopentyl methyl ether (CPME)

75746

Sigma-Aldrich

Sodium dodecyl sulfate, ≥99.0% (GC), dust-free pellets

204439

Sigma-Aldrich

Sodium chloride, 99.999% trace metals basis

378860

Sigma-Aldrich

Sodium chloride, random crystals, 99.9% trace metals basis

436143

Sigma-Aldrich

Sodium dodecyl sulfate, ACS reagent, ≥99.0%

451940

Sigma-Aldrich

Sodium chloride-³⁵Cl, 99 atom % ³⁵Cl

S0817

Sigma-Aldrich

Sodium chloride solution, 0.85%

63069

Sigma-Aldrich

Magnesium chloride solution, BioUltra, Molecular Biology, ~1 M in H₂O

71726

Supelco

Sodium dodecyl sulfate, suitable for ion pair chromatography, LiChropur^™, ≥99.0%

62862

Sigma-Aldrich

Sodium dodecyl sulfate, ≥90% ((Assay))

71725

Sigma-Aldrich

Sodium dodecyl sulfate, BioUltra, Molecular Biology, ≥99.0% (GC)

450006

Sigma-Aldrich

Sodium chloride, AnhydroBeads^™, −10 mesh, 99.999% trace metals basis

71376

Sigma-Aldrich

Sodium chloride, BioUltra, Molecular Biology, ≥99.5% (AT)

17934

Supelco

Hydrogen chloride – ethanol, ~1.25 M HCl, derivatization grade (GC derivatization), LiChropur^™

71386

Sigma-Aldrich

Sodium chloride solution, BioUltra, Molecular Biology, ~5 M in H₂O

71383

Sigma-Aldrich

Sodium chloride, tested according to Ph. Eur.

17933

Supelco

Hydrogen chloride – 2-propanol solution, ~1.25 M HCl (T), derivatization grade (GC derivatization), LiChropur^™

71729

Sigma-Aldrich

Sodium dodecyl sulfate, ≥98.0% (GC)

63036

Sigma-Aldrich

Magnesium chloride solution, BioUltra, Molecular Biology, ~0.025 M in H₂O

68475

Sigma-Aldrich

Magnesium chloride solution, BioUltra, Molecular Biology, 2 M in H₂O

71387

Supelco

Sodium chloride, reference material for titrimetry, certified by BAM, >99.5%

84429

Sigma-Aldrich

Hydrochloric acid solution, ~6 M in H₂O, for amino acid analysis

17935

Supelco

Hydrogen chloride – methanol solution, ~1.25 m HCl (T), derivatization grade (GC derivatization), LiChropur^™

S5886

Sigma-Aldrich

Sodium chloride, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99%

S7653

Sigma-Aldrich

Sodium chloride, BioXtra, ≥99.5% (AT)

S6150

Sigma-Aldrich

Sodium chloride, tablet

S8776

Sigma-Aldrich

Sodium chloride solution, 0.9% in water, BioXtra, suitable for cell culture

S1679

Sigma-Aldrich

Sodium chloride, meets analytical specification of Ph. Eur., BP, USP, 99.0-100.5%

S3014

Sigma-Aldrich

Sodium chloride, Molecular Biology, DNase, RNase, and protease, none detected, ≥99% (titration)

L3771

Sigma-Aldrich

Sodium dodecyl sulfate, BioReagent, suitable for electrophoresis, Molecular Biology, ≥98.5% (GC)