shRNA Gene Families Sets
The MISSION® shRNA Library gene family sets are collections of genes related to specific cellular/molecular pathways documented in NCBI, Gene Ontology (GO), Protein Lounge, and Ingenuity Systems. Our Bioinformatics team has methodically mapped all TRC shRNA clones to each set. Gene family sets are fully customizable allowing for complete flexibility to add or delete clone or gene content. Additionally, any panel can be arrayed in either 96- or 384-well plate formats. All of the gene family sets can be used at the benchtop for focused target discovery and pathway analysis.
Formats Available
Format | Material Provided | Quality Control |
---|---|---|
Bacterial Glycerol Stock | 50 µl aliquot of bacterial glycerol stocks (terrific broth, carbenicillin at 100 µg/ml and 15% glycerol) | Sequencing Verification |
Purified Plasmid DNA | A minimum of 1 µg of purified plasmid DNA is provided and is aliquoted at 40 µl per well | Sequencing Verification DNA Quantitation |
Lentivirus (representational, plate titer) | 4 x 50 µl aliquots of VSV-G pseudotyped lentivirus, 10% representational plate titer | Sequencing Verification p24 ELISA Titer |
Lentivirus (full, individual well titer) | 4 x 50 µl aliquots of VSV-G pseudotyped lentivirus, fully titered to individual wells | Sequencing Verification p24 ELISA Titer |
Mission shRNA Gene Family Sets
Gene Family Set | Species | Product Numbers | Product Information | |||||
---|---|---|---|---|---|---|---|---|
Glycerol | Plasmid DNA | Lentivirus Rep. Titer | Lentivirus Full Titer | Gene Count | Clone Count | Avg Number Clone/Gene | ||
Apoptosis Pathway | Human | SH1911 | SH1921 | SH1931 | inquire | 441 | 3520 | 8.0 |
B-Cell Activation | Human | SH2911 | SH2921 | SH2931 | inquire | 99 | 661 | 6.7 |
Cell Adhesion Genes | Human | SH2211 | SH2221 | SH2231 | inquire | 366 | 2396 | 6.5 |
Cytokine and Chemokine | Human | SH0811 | SH0821 | SH0831 | inquire | 106 | 528 | 5.0 |
Cytokine and Chemokine Receptors | Human | SH1311 | SH1321 | SH1331 | inquire | 93 | 584 | 6.3 |
Cytoskeleton Genes | Human | SH2311 | SH2321 | SH2331 | inquire | 273 | 1991 | 7.3 |
Epigenetic Regulators | Human | SH3011 | SH3021 | SH3031 | inquire | 11 | 57 | 5.2 |
DNA Repair Pathway | Human | SH1811 | SH1821 | SH1831 | inquire | 117 | 837 | 7.2 |
Ubiquitin Hydrolases (DUBS) | Human | SH0711 | SH0721 | SH0731 | inquire | 124 | 818 | 6.6 |
Extracellular Matrix Genes | Human | SH2511 | SH2521 | SH2531 | inquire | 330 | 1968 | 6.0 |
G-Protein Coupled Receptors (GPCRs) | Human | SH0211 | SH0221 | SH0231 | inquire | 542 | 2864 | 5.3 |
Helicase | Human | SH2611 | SH2621 | SH2631 | inquire | 133 | 909 | 6.8 |
Ion Channel | Human | SH1011 | SH1021 | SH1031 | inquire | 278 | 1456 | 5.2 |
JAK-STAT Pathway | Human | SH2711 | SH2721 | SH2731 | inquire | 190 | 1309 | 6.9 |
Kinases, complete | Human | SH0111 | SH0121 | SH0131 | inquire | 746 | 9418 | 12.6 |
Nuclear Hormone Receptors | Human | SH1111 | SH1121 | SH1131 | inquire | 216 | 1448 | 6.7 |
p53 Pathway | Human | SH2411 | SH2421 | SH2431 | inquire | 240 | 1865 | 7.8 |
Phosphatases | Human | SH0411 | SH0421 | SH0431 | inquire | 317 | 2065 | 6.5 |
T-Cell Activation | Human | SH2811 | SH2821 | SH2831 | inquire | 241 | 1469 | 6.1 |
Tumor Supressors | Human | SH0511 | SH0521 | SH0531 | inquire | 73 | 575 | 7.9 |
Tyrosine Kinases | Human | inquire | inquire | inquire | inquire | 133 | 1728 | 13.0 |
Ubiquitin Ligases (E1, E2, E3) | Human | SH2111 | SH2121 | SH2131 | inquire | 341 | 2081 | 6.1 |
Kinases, complete | Mouse | SM0111 | SM0121 | SM0131 | inquire | 598 | 3916 | 6.5 |
Phosphatase | Mouse | SM0411 | SM0421 | SM0431 | inquire | 286 | 1734 | 6.1 |
Tyrosine Kinase | Mouse | SM2011 | SM2021 | SM2031 | inquire | 130 | 850 | 6.5 |
*The MISSION production and bio-informatics team constantly reviews and quality controls clones available for a gene family set. These numbers are very close to the actual number that will be shipped, but each researcher will receive a final plate map indicating the location and exact TRCN clone numbers.
If your gene set of interest is not available, please inquire for custom preparation at [email protected]. We can assemble custom gene sets at any time.
Contact Us
For questions about the library, pricing and quotes or other concerns, please e-mail us at: [email protected].
MISSION is a registered trademark of Sigma-Aldrich Co. LLC Label License.
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