2,2′-azino-di-(3-ethylbenzthiazoline sulfonic acid) or ABTS™
acts a substrate for HRP (horseradish peroxidase) conjugate during enzyme-linked immunosorbent assay (ELISA). It is the most sensitive, and stable substrate when compared to three other substrates namely, 5-aminosalicylic acid (5AS), O
-phenylenediamine (OPD), O
-tolidine (OT). It also produces the best visual results, where it gives a bluish-green color. ELISA using ABTS™
is a highly sensitive, specific and reproducible technique
This solution is an excellent substrate for enzyme immunoassays with horseradish peroxidase as a marker enzyme.