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Nitrite/Nitrate, Colorimetric Test

sufficient for ~64 assays, pkg of 1 tests (5 components), suitable for water monitoring

Nitrate, Nitrite, colormetric test


sufficient for ~64 assays


pkg of 1 tests (5 components)




water monitoring

storage temp.


General description

The method is linear in the range from 0.02 mg nitrite or nitrate/l sample solution to 5.00 mg nitrite or nitrate/l sample solution.

Detection limit: 0.02mg/l for nitrite and nitrate


Under the provided conditions, nitrate reductase reacts specifically with nitrate ions.


Semimicro method for the determination of nitrite and/or nitrate to investigate water, waste water, environmental samples, plant material, foodstuffs, drugs, cosmetics, and biological samples. Nitrite/Nitrate, Colorimetric Test was used in a study done to estimate the in vivo effects of myrtle oil (Myrtii oleum) on the antioxidant enzymes such as catalase and superoxide dismutase in alloxan and normoglycaemic diabetic rabbits.


1 test combination containing 5 components.

Preparation Note

Working solution: Preparation of the solutions
  • Use the contents of bottle #1 undiluted, bring to 15 to 25 °C before use.
  • Place one Coenzyme-tablet in a beaker and dissolve in 3 ml of the solution from bottle 1. Remove the Coenzyme-tablet using the tweezers provided. The resulting solution is reaction mixture 2 and is sufficient for 12 nitrate determinations, bring to 15 to 25 °C before use.
  • Dissolve the contents of one bottle #3 in 0.7 ml of double-dist. water to give solution 3.
  • Use the contents of one bottle #4 undiluted.
  • Use the contents of one bottle #5 undiluted.

Storage conditions (working solution):
  • Prepare Reaction Mixture 2 immediately before use.
  • Solution of Nitrate reductase is stable at 2 to 8 °C for 2 weeks.
  • Solution of Color reagent I is stable at 2 to 8 °C for 3 months.
  • Solution of Color reagent II is stable at 2 to 8 °C for 3 months.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Kit Components Only

Product No.

  • Potassium phosphate buffer, stabilized, pH 7.5

  • NADPH, tablets, each containing NADPH and stabilizers

  • Nitrate reductase, lyophilized, 8 U

  • Color Reagent I, consisting of sulfanilamide and stabilizers

  • Color Reagent II, consisting of N-(1-naphthyl)-ethylenediamine dihydrochloride and stabilizers


CorrosionHealth hazard

Signal Word


Hazard Statements

Hazard Classifications

Eye Dam. 1 - Met. Corr. 1 - Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

8B - Non-combustible, corrosive hazardous materials



Flash Point(F)

does not flash

Flash Point(C)

does not flash

Certificate of Analysis

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Certificate of Origin

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Product Information Sheet

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