23479

Nitrite/Nitrate Assay Kit, colorimetric

used for detection of nitric oxide metabolites

• Synonym(s): Nitrate/Nitrite Assay Kit, colorimetric
• NACRES: NA.84

Properties

• Quality Level: 100
• usage: sufficient for 100 assays
• suitability: passes test for application
• storage temp.: 2-8°C

Description

Application

Nitric oxide (NO), a gaseous paramagnetic radical, is a very important and versatile messenger in biological systems. NO is synthesized from L-arginine by NO synthase (NOS). It has been identified as an endothelial derived relaxation factor (EDRF) and antiplatelet substance. It serves as a neurotransmitter derived from a neutrophile and a cytotoxic substance from an activated macrophage. Although NO′s molecular action in the biological system is very versatile, the most important role of NO is the activation of guanylate cyclase.

The Griess assay is one of the most popular and simplest methods used to detect the NO concentration. The Griess assay′s mechanism is summarized as the azo coupling between diazonium species, which are produced from sulfanilamide with NO₂, and naphthylethylenediamine. The NO₂/NO₃ Assay Kit contains these dyes, a nitrate reductase, an enzyme co-factor, buffer solution and NO₂, NO₃ solutions as standards. Therefore, total NO metabolites are easily detectable using this kit. The suitable NO₂ detection range is from 10 to 100 μM.

Kit Components Only

• NaNO₂ Standard Solution (100 mM) 1 bottle
• NaNO₃ Standard Solution (100 mM) 1 bottle
• Buffer solution (20 mM, pH 7.6) 1 bottle
• Nitrate reductase (lyophilized) 1 bottle
• Enzyme Co-factor (lyophilized) 1 bottle
• Griess Reagent A 1 bottle
• Griess Reagent B 1 bottle

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H315 - H319
• Precautionary Statements: P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313 - P337 + P313
• Hazard Classifications: Eye Irrit. 2 - Skin Irrit. 2
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable