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Nitrite/Nitrate Assay Kit, colorimetric

used for detection of nitric oxide metabolites

Nitrate/Nitrite Assay Kit, colorimetric

Quality Level


sufficient for 100 assays


passes test for application

storage temp.



Nitric oxide (NO), a gaseous paramagnetic radical, is a very important and versatile messenger in biological systems. NO is synthesized from L-arginine by NO synthase (NOS). It has been identified as an endothelial derived relaxation factor (EDRF) and antiplatelet substance. It serves as a neurotransmitter derived from a neutrophile and a cytotoxic substance from an activated macrophage. Although NO′s molecular action in the biological system is very versatile, the most important role of NO is the activation of guanylate cyclase.

The Griess assay is one of the most popular and simplest methods used to detect the NO concentration. The Griess assay′s mechanism is summarized as the azo coupling between diazonium species, which are produced from sulfanilamide with NO2, and naphthylethylenediamine. The NO2/NO3 Assay Kit contains these dyes, a nitrate reductase, an enzyme co-factor, buffer solution and NO2, NO3 solutions as standards. Therefore, total NO metabolites are easily detectable using this kit. The suitable NO2 detection range is from 10 to 100 μM.

Kit Components Only

Product No.

  • NaNO2 Standard Solution (100 mM) 1 bottle

  • NaNO3 Standard Solution (100 mM) 1 bottle

  • Buffer solution (20 mM, pH 7.6) 1 bottle

  • Nitrate reductase (lyophilized) 1 bottle

  • Enzyme Co-factor (lyophilized) 1 bottle

  • Griess Reagent A 1 bottle

  • Griess Reagent B 1 bottle


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Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Storage Class Code

10 - Combustible liquids



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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