Merck
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90101

Sigma-Aldrich

Ampliflu Red

for fluorescence, ≥98.0% (HPLC)

Synonym(s):
10-Acetyl-3,7-dihydroxyphenoxazine
Empirical Formula (Hill Notation):
C14H11NO4
CAS Number:
Molecular Weight:
257.24
MDL number:
PubChem Substance ID:
NACRES:
NA.32

grade

for fluorescence

Quality Level

Assay

≥98.0% (HPLC)

form

powder

fluorescence

λex 571 nm; λem 585 nm in DMSO

storage temp.

2-8°C

SMILES string

CC(=O)N1c2ccc(O)cc2Oc3cc(O)ccc13

InChI

1S/C14H11NO4/c1-8(16)15-11-4-2-9(17)6-13(11)19-14-7-10(18)3-5-12(14)15/h2-7,17-18H,1H3

InChI key

PKYCWFICOKSIHZ-UHFFFAOYSA-N

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Ampliflu™ Red for fluorescence, ≥98.0% (HPLC)

Sigma-Aldrich

90101

Ampliflu Red

Sudan Red 7B for microscopy (Bot., Hist.)

Sigma-Aldrich

46290

Sudan Red 7B

Nuclear Fast Red for microscopy (Bot., Hist.)

Sigma-Aldrich

60700

Nuclear Fast Red

m-Cresol Purple indicator grade, Dye content 90 %

Sigma-Aldrich

857890

m-Cresol Purple

grade

for fluorescence

grade

for microscopy (Bot., Hist.)

grade

for microscopy (Bot., Hist.)

grade

indicator grade

assay

≥98.0% (HPLC)

assay

-

assay

-

assay

-

form

powder

form

powder

form

powder

form

powder

fluorescence

λex 571 nm; λem 585 nm in DMSO

fluorescence

-

fluorescence

-

fluorescence

-

storage temp.

2-8°C

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

200

Application

Ampliflu Red has been used to study mitochondrial abnormalities and oxidative stress related to Parkinson’s Disease. Ampliflu Red assay was suitable as an alternative indicator assay for detecting the inhibition of hTPO (human thyroid peroxidase) activity. Phospholipase D, an essential cellular process ingredient, used Ampliflu Red (10-Acetyl-3,7-dihydroxyphenoxazine) to determine its activity.Ampliflu Red is used for the following applications:
  • Quantification of inflammatory mediators
  • Determination of free and esterified cholesterol contents
  • Evaluation of compounds as substrates for GABA-AT compounds

Legal Information

Ampliflu is a trademark of Sigma-Aldrich Co. LLC

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Precautionary Statements

Hazard Classifications

Acute Tox. 4 Oral

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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