D0550

3,5-Dinitrosalicylic acid

used in colorimetric determination of reducing sugars

• Synonym(s): 3,5-Dinitro-2-hydroxybenzoic acid, DNS
• Linear Formula: (O₂N)₂C₆H₂-2-(OH)CO₂H
• CAS Number: 609-99-4
• Molecular Weight: 228.12
• Beilstein: 2220661
• EC Number: 210-204-3
• MDL number: MFCD00007104
• PubChem Substance ID: 24893243

Properties

• Quality Level: 200
• Assay: ≥98% (HPLC and titration)
• form: powder
• mp: 168-172 °C (lit.)
• solubility: H₂O: 50 mg/mL
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: OC(=O)c1cc(cc(c1O)[N+]([O-])=O)[N+]([O-])=O
• InChI: 1S/C7H4N2O7/c10-6-4(7(11)12)1-3(8(13)14)2-5(6)9(15)16/h1-2,10H,(H,11,12)
• InChI key: LWFUFLREGJMOIZ-UHFFFAOYSA-N

Description

Application

3,5-Dinitrosalicylic acid (DNS) has been used to stop the reaction in in vitro α-amylase inhibition study. It has also been used for the quantitation of enzymatically released reducing sugars.

Biochem/physiol Actions

3,5-Dinitrosalicylic acid (DNS) is used in colorimetric determination of reducing sugars and to analyze glycosidase (glycoside hydrolase) activity by quantitation of enzymatically released reducing sugar. The dinitrosalicylic acid method has been compared to the Nelson-Somogi colorimetric method.

Safety Information

• Pictograms: GHS02,GHS07
• Signal Word: Danger
• Hazard Statements: H228 - H302
• Precautionary Statements: P210 - P240 - P241 - P264 - P270 - P301 + P312
• Hazard Classifications: Acute Tox. 4 Oral - Flam. Sol. 1
• Storage Class Code: 4.1A - Other explosive hazardous materials
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves