Merck
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MAK058

Sigma-Aldrich

D-Lactate Colorimetric Assay

sufficient for 100 colorimetric tests

NACRES:
NA.84

usage

sufficient for 100 colorimetric tests

detection method

colorimetric

storage temp.

−20°C

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D-Lactate Assay Kit sufficient for 100 colorimetric tests

Sigma-Aldrich

MAK336

D-Lactate Assay Kit

Lactate Assay Kit II sufficient for 100 colorimetric tests

Sigma-Aldrich

MAK065

Lactate Assay Kit II

L-Lactate Assay Kit sufficient for 100 colorimetric tests

Sigma-Aldrich

MAK329

L-Lactate Assay Kit

usage

sufficient for 100 colorimetric tests

usage

sufficient for 100 colorimetric tests

usage

sufficient for 100 colorimetric tests

usage

sufficient for 100 colorimetric tests

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

General description

D-Lactate, typically present in the bloodstream at nanomolar concentrations, is produced by an intestinal source or via the methylglyoxal pathway. In mammals, D-Lactate metabolism requires D-Lactate hydrogenase and is metabolized slowly, thus an increase in blood concentration levels can lead to acidemia and acidosis. The severity of this D-lactic acidosis can be associated with neurotoxic symptoms. Significant D-Lactate accumulations in the body can also be related to impaired metabolism and excretion.

Application

D-Lactate Colorimetric Assay kit has been used to determine the stereospecificity of lactate produced.

Suitability

Suitable for use with samples of serum, plasma, cells, culture and fermentation media.

Principle

In this assay, D-Lactate is specifically oxidized by D-Lactate hydrogenase and generates a proportional colorimetric product measured at 450 nm. The useful concentration range in samples is 0.1-10 mM D-Lactate.

Pictograms

Corrosion

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Dam. 1 - Skin Corr. 1B

Storage Class Code

8A - Combustible, corrosive hazardous materials

WGK

WGK 3

Certificate of Analysis

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Certificate of Origin

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Product Information Sheet

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