MAK064

Lactate Assay Kit

sufficient for 100 colorimetric or fluorometric tests

• Synonym(s): Lactic Acid Assay Kit
• NACRES: NA.84

Properties

• usage: sufficient for 100 colorimetric or fluorometric tests
• detection method: colorimetric; fluorometric
• storage temp.: −20°C

Description

General description

L(+)-Lactate is a metabolic compound formed in animals by the action of the enzyme Lactate Dehydrogenase. Lactate is produced in proliferating cells and during anaerobic conditions such as exercise. Abnormally high concentrations of lactate have been related to pathological conditions such as cancer, diabetes, and lactate acidosis. L(+)-Lactate is the major stereoisomer of lactate formed in human intermediary metabolism and is present in blood at levels of around 1-2 mmol/L. This kit provides a convenient means for detecting L(+)-Lactate in biological samples. There is no need of pretreatment or purification of samples.

Application

Lactate Assay Kit has been used to determine the concentrations of lactate in samples.

Suitability

Suitable for detecting L(+)-Lactate in culture medium, fermentation medium, blood and cells.

Principle

In this assay, lactate concentration is determined by an enzymatic assay, which results in a colorimetric (570 nm)/ fluorometric (λ_ex = 535 nm/λe_m = 587 nm) product, proportional to the lactate present. Typical levels of sensitivity for this kit are between 0.001-10 mM.

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H317 - H334
• Precautionary Statements: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
• Hazard Classifications: Resp. Sens. 1 - Skin Sens. 1
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): 188.6 °F - closed cup
• Flash Point(C): 87 °C - closed cup