MAK164

Intracellular Hydrogen Peroxide Assay

sufficient for 200 fluorometric tests (green fluorescence)

• EC Number: 200-664-3
• NACRES: NA.84

Properties

• usage: sufficient for 200 fluorometric tests (green fluorescence)
• detection method: fluorometric
• storage temp.: −20°C

Description

General description

Hydrogen peroxide, a reactive oxygen species produced through the metabolism of molecular oxygen, serves as both an intracellular signaling messenger and a source of oxidative stress. Hydrogen peroxide is generated in cells via multiple mechanisms such as the NOX-mediated ROS production by neutrophils and macrophages (respiratory burst) or by the dismutase of superoxide anions produced as a result of electron leak during mitochondrial respiration. Abnormal hydrogen peroxide production contributes to oxidative cell damage and the progression of diseases such as asthma, atherosclerosis, osteoporosis, and neurodegeneration.

Application

Intracellular hydrogen peroxide assay kit has been used to measure intracellular hydrogen peroxide levels.

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

This kit is suitable for the detection of hydrogen peroxide in living cells and a variety of samples such as cell extracts and liquids.

Principle

The Intracellular Hydrogen Peroxide Assay Kit provides a simple and reproducible method to quantify hydrogen peroxide levels in living cells and in a variety of other samples such as cellular extracts. This kit utilizes a unique cell-permeable sensor that generates a fluorescent product (λ_ex = 490/λ_em = 520 nm) after reaction with hydrogen peroxide that can be analyzed by a fluorescent microplate reader, flow cytometer, or fluorescent microscope.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3