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Key Documents

MWND500

Sigma-Aldrich

Kit for Molecular Weights 14,000-500,000 Non-denaturing

Synonym(s):

protein markers, protein molecular weight standards, protein standards

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About This Item

UNSPSC Code:
41105337
NACRES:
NA.32

form

lyophilized powder

storage temp.

−20°C

Application

Kit for Molecular Weights 14,000-500,000 Non-denaturing has been used as a standard for non-denaturing PAGE (polyacrylamide gel electrophoresis) analysis. It has also been used to calibrate columns for gel exclusion chromatography.

Packaging

The kit contains 5 vials of proteins (1 mg of each) having a molecular weight range of 14 - 545 kDa and a technical bulletin.

Proteins:
α-Lactalbumin from bovine milk
Carbonic Anhydrase from bovine erythrocytes
Albumin from chicken egg white
Albumin from bovine serum
Urease from Jack bean

Reconstitution

Reconstitute the contents of each vial of protein marker with 1ml of water, with the exception of Urease, Catalog Number U7752. Reconstitute the vial of Urease with 0.5 ml of water and mix until the protein is in solution. Then add 0.5 ml of glycerol to the Urease solution to stabilize the protein during freeze thaw cycles. Solutions may be stored at -20 °C for future use. It is recommended that repeated freezing and thawing be avoided. Stock solutions may be dispensed into working aliquots, frozen, and then discarded after 2-3 uses. Immediately before use, thaw an aliquot of each prepared protein marker and dilute with an equal volume of sample buffer.

Pictograms

Health hazardExclamation mark

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Assembly of phagocyte NADPH oxidase: A concerted binding process?
Gilda Karimi
Biochim. Biophys. Acta Gen. Subj. (2014)
Immunogenicity and Serological Cross-Reactivity of Saliva Proteins among Different Tsetse Species
Xin Zhao
PLoS ONE, e0004038-e0004038 (2015)
Oligomerization of the microtubule-associated protein tau is mediated by its N-terminal sequences: implications for normal and pathological tau action
H. Eric Feinstein
Journal of Neurochemistry (2016)
Costas Delis et al.
RNA biology, 13(1), 68-82 (2015-12-01)
We report the identification and characterization of a novel gene, AtHesperin (AtHESP) that codes for a deadenylase in Arabidopsis thaliana. The gene is under circadian clock-gene regulation and has similarity to the mammalian Nocturnin. AtHESP can efficiently degrade poly(A) substrates
Gilda Karimi et al.
Biochimica et biophysica acta, 1840(11), 3277-3283 (2014-08-12)
The phagocyte NADPH-oxidase is a multicomponent enzyme that generates superoxide anions. It comprises a membrane redox component flavocytochrome b558 and four cytosolic proteins (p67(phox), p47(phox), p40(phox) and Rac) that must assemble to produce an active system. In this work we

Articles

Explore the impact of water quality on protein electrophoresis and Western blotting. Learn about the role of ultrapure water and its effect on chemiluminescent signals in Western blotting.

Protocols

Separation of various acids and compounds by molecular weight: PAA, PEI, PIDADMACl, PAS, cationic dextran, chitosan

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