Merck
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O3514

Sigma-Aldrich

Osteopontin from bovine milk

lyophilized powder

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Synonym(s):
Bone Sialoprotein-1, Early T Lymphocyte Activation Protein-1 (ETA-1), OPN, Secreted Phosphoprotein 1 (Spp1)
MDL number:
MFCD03100698
NACRES:
NA.32

biological source

bovine milk

Quality Level

100

sterility

sterile

form

lyophilized powder

mol wt

~60 kDa

packaging

pkg of 50 μg

technique(s)

cell culture | mammalian: suitable

UniProt accession no.

P31098
P31096

shipped in

ambient

storage temp.

−20°C

Gene Information

cow ... SPP1(281499)

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Osteopontin from bovine milk

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Osteopontin human

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SRP3131

Osteopontin human

κ-Casein from bovine milk ≥70% (PAGE), lyophilized powder

Sigma-Aldrich

C0406

κ-Casein from bovine milk

biological source

bovine milk

biological source

human

biological source

human

biological source

bovine milk

sterility

sterile

sterility

sterile

sterility

-

sterility

-

form

lyophilized powder

form

lyophilized powder

form

lyophilized

form

lyophilized powder

mol wt

~60 kDa

mol wt

60-65 kDa by SDS-PAGE (reducing)

mol wt

60.0-65.0 kDa

mol wt

-

packaging

pkg of 50 μg

packaging

pkg of 50 μg

packaging

pkg of 50 μg

packaging

-

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

isoelectric focusing (IEF): suitable

General description

Osteopontin (OPN) is a multifunctional protein. It is released by macrophages, T cells and epithelial cells. It has 261 amino acid residues with a molecular mass of 29,283 Da. Osteopontin is also called as uropontin. Osteopontin from bovine milk is highly glycosylated and phosphorylated. SPP1 gene (secreted phosphoprotein 1) codes for osteopontin. This gene is located on human chromosome 4 and cow chromosome 6. It has seven exons and six introns.

Biochem/physiol Actions

A secreted, highly acidic, calcium binding, phosphorylated glycoprotein that is chemotactic for macrophages, smooth muscle cells, endothelial cells, and glial cells. In activated macrophages, OPN inhibits nitric oxide production and cytotoxicity. Human, mouse, rat, pig, and bovine OPN share 40% amino acid sequence identity.
Osteopontin stimulates cell mediated responses, chemotaxis of inflammatory cells and anti-inflammatory responses. It binds to av-containing integrins and stimulate cell adhesion and migration. Osteopontin promotes Th1 type immunity.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 2.5 mg bovine serum albumin.

Analysis Note

The biological activity is measured by its ability to mediate 293 cell adhesion.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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