Horseradish peroxidase (HRP) is isolated from horseradish roots (Amoracia rusticana
). It is used in biochemistry applications such as western blots, ELISA and Immunohistochemistry. Horseradish peroxidase is used to amplify a weak signal and increase detectability of a target molecule, such as a protein. Product P6782 is type VI-A and it is essentially a salt-free, lyophilized powder. It is used to quantify cholesterol and cholesterol esters
and to study in vitro
lipid deposition on hydrogel and silicone hydrogel contact lenses
The enzyme from Sigma has been used in the development of rapid and sensitive galactose oxidase-peroxidase biosensor for galactose detection with prolonged stability.
It has been used as a component of a media (MRS agar) plate containing 3,3′,5,5′-tetramethylbenzidine (TMB) for the growth of Lactobacillus
sp. TMB acts as a chromogenic substrate of peroxidase. Peroxidase generates O2
produced by the lactobacilli
, and the TMB stains the colonies blue in the presence of O2
. Thus, after 48 hours of incubation under 5% CO2
in air, the colonies that produce H2
on MRS agar appear dark blue. H2
nonproducers are colorless.
It has been used to study the stabilization effect of polyvinyl alcohol on horseradish peroxidase.
It is also used for the determination of glucose