S0389

Sucrose

for molecular biology, ≥99.5% (GC)

• Synonym(s): α-D-Glc-(1→2)-β-D-Fru, α-D-Glucopyranosyl β-D-fructofuranoside, β-D-Fructofuranosyl-α-D-glucopyranoside, D(+)-Saccharose, Sugar
• Empirical Formula (Hill Notation): C₁₂H₂₂O₁₁
• CAS Number: 57-50-1
• Molecular Weight: 342.30
• Beilstein: 90825
• EC Number: 200-334-9
• MDL number: MFCD00006626
• PubChem Substance ID: 24899423
• NACRES: NA.31

Properties

• biological source: sugar cane
• Quality Level: 300
• grade: for molecular biology
• Assay: ≥99.5% (GC)
• form: crystals
• technique(s): gas chromatography (GC): suitable
• impurities: ≤0.1% free glucose
• mp: 185-187 °C (lit.)
• solubility: H₂O: soluble 50 g + 50 mL
• cation traces: heavy metals (as Pb): ≤5 ppm
• absorption: ≤0.15 at 280 at 50%; ≤0.20 at 260 at 50%
• application(s): agriculture
• foreign activity: DNase, RNase, protease, none detected
• SMILES string: OC[C@H]1O[C@H](O[C@]2(CO)O[C@H](CO)[C@@H](O)[C@@H]2O)[C@H](O)[C@@H](O)[C@@H]1O
• InChI: 1S/C12H22O11/c13-1-4-6(16)8(18)9(19)11(21-4)23-12(3-15)10(20)7(17)5(2-14)22-12/h4-11,13-20H,1-3H2/t4-,5-,6-,7-,8+,9-,10+,11-,12+/m1/s1
• InChI key: CZMRCDWAGMRECN-UGDNZRGBSA-N

Description

General description

Sucrose, an abundant disaccharide, is the end product of photosynthesis. This nonreducing sugar is composed of glucose and fructose. It is obtained from cane sugar. Sucrose is predominantly synthesized in leaves.

Application

Sucrose has been used:

• to perform cryoprotection for kidney tissues
• as a component in the solid germination medium to germinate pollen grains
• in the triphenyl-tetrazolium chloride (TTC) test to check the viability of pollen grains
• in the cryoprotection of fixed brain tissues for immunohistochemistry

Biochem/physiol Actions

Sucrose is used as a transport and storage molecule in most plants due to its limited chemical reactivity. It can be used to prepare density gradients for the separation of cell/organelle. Sucrose can be used in the plant, insect, and bacterial culture media as a supplement. In addition, sucrose can also be used in several enzymatic assays.

Features and Benefits

• DNase, RNase, and protease-free     
• Not synthetic   
• For molecular biology applications

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves