S7903

Sucrose

BioXtra, ≥99.5% (GC)

• Synonym(s): α-D-Glc-(1→2)-β-D-Fru, α-D-Glucopyranosyl β-D-fructofuranoside, β-D-Fructofuranosyl-α-D-glucopyranoside, D(+)-Saccharose, Sugar
• Empirical Formula (Hill Notation): C₁₂H₂₂O₁₁
• CAS Number: 57-50-1
• Molecular Weight: 342.30
• Beilstein: 90825
• EC Number: 200-334-9
• MDL number: MFCD00006626
• PubChem Substance ID: 24899768
• NACRES: NA.21

Properties

• biological source: sugar cane
• Quality Level: 300
• product line: BioXtra
• Assay: ≥99.5% (GC)
• form: crystals
• optical activity: [α]20/D +66.5±1°, c = 10 in H₂O(lit.)
• technique(s): gas chromatography (GC): suitable
• impurities: Insoluble matter, passes filter test
• ign. residue: ≤0.01% (as SO₄)
• loss: ≤0.2% loss on drying, HV, 18 - 26°C
• pH: 5.5-7.5 (20 °C, 1 M in H₂O)
• mp: 185-187 °C (lit.)
• solubility: H₂O: 1 M, clear, colorless
• anion traces: chloride (Cl^-): ≤0.005%; sulfate (SO₄^2-): ≤0.005%
• cation traces: Al: ≤0.0005%; As: ≤0.0001%; Ba: ≤0.0005%; Bi: ≤0.0005%; Ca: ≤0.001%; Cd: ≤0.0005%; Co: ≤0.0005%; Cr: ≤0.0005%; Cu: ≤0.0005%; Fe: ≤0.0005%; K: ≤0.005%; Li: ≤0.0005%; Mg: ≤0.0005%; Mn: ≤0.0005%; Mo: ≤0.0005%; Na: ≤0.005%; Ni: ≤0.0005%; Pb: ≤0.0005%; Sr: ≤0.0005%; Zn: ≤0.0005%
• absorption: ≤0.07 at 280 in H₂O at 1 M; ≤0.09 at 260 in H₂O at 1 M
• SMILES string: OC[C@H]1O[C@H](O[C@]2(CO)O[C@H](CO)[C@@H](O)[C@@H]2O)[C@H](O)[C@@H](O)[C@@H]1O
• InChI: 1S/C12H22O11/c13-1-4-6(16)8(18)9(19)11(21-4)23-12(3-15)10(20)7(17)5(2-14)22-12/h4-11,13-20H,1-3H2/t4-,5-,6-,7-,8+,9-,10+,11-,12+/m1/s1
• InChI key: CZMRCDWAGMRECN-UGDNZRGBSA-N

Description

Application

Sucrose may be used to prepare density gradients for cell/organelle separation. The refractive index of the solution will be directly related to the density. In addition, sucrose can be used as a supplement in plant, insect, and bacterial culture media, and in various enzymatic assays.

Preparation Note

Solutions can be autoclaved for 15-20 minutes at a maximum of 121 °C. There will be some hydrolysis to glucose and fructose, depending in part on how rapidly the autoclave comes to the required temperature and pressure. Care must be taken to prevent solutions from caramelizing.

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves