55269-U
HybridSPE®-Phospholipid Ultra solid phase extraction (SPE) Cartridge
cartridge, bed wt. 30 mg, volume 1 mL, pk of 100
Synonym(s):
HybridSPE®-Phospholipid
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 100 ea | Please contact Customer Service for Availability | $440.00 |
About This Item
Product Name
HybridSPE®-Phospholipid Ultra, cartridge, bed wt. 30 mg, volume 1 mL, pk of 100
Quality Level
material
PE frit (5-9 μm), polypropylene hardware
composition
bed wt., 30 mg
packaging
pk of 100
technique(s)
solid phase extraction (SPE): suitable
volume
1 mL
matrix active group
zirconia-based phase
General description
The "In-well" and "In-cartridge" precipitation methods are available for the HybridSPE-Phospholipid 96-well version and HybridSPE-Phospholipid Ultra cartridge in which biological plasma/serum is first added to either the well or cartridge, followed by acidified acetonitrile (precipitation agent). After a brief mixing/vortexing step, vacuum is applied. Because the 96-well and Ultra cartridge versions contain a series of low porosity hydrophobic filters/frits, the packed-bed filter/frit assembly acts as a depth filter facilitating the concurrent removal of both phospholipids and precipitated proteins during the extraction process. Standard HybridSPE-Phospholipid cartridges require an "off-line" precipitation method.
Features and Benefits
- Merges the simplicity of protein precipitation and the selectivity of SPE via the targeted removal of phospholipids
- Reduce ion-suppression through the complete removal of phospholipids and precipitated proteins
- 2-3 step generic procedure
- Minimal to no method development
- Available in 96-well and 1 mL cartridge dimensions
- Ultra cartridge allows the use of "In-cartridge" protein precipitation method
- Filtrate is ultra clean, free of fines, and suitable for direct injection in UHPLC-type applications
Legal Information
1 of 1
This Item | |||
|---|---|---|---|
| matrix active group zirconia-based phase | matrix active group zirconia-based phase | matrix active group zirconia-based phase | matrix active group zirconia-based phase |
| material PE frit (5-9 μm), polypropylene hardware | material - | material - | material PE frit (20 μm), polypropylene hardware |
| volume 1 mL | volume 0.8 mL | volume 0.8 mL | volume 1 mL |
| composition bed wt., 30 mg | composition bed wt., 15 mg | composition bed wt., 15 mg | composition bed wt., 30 mg |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
| packaging pk of 100 | packaging pk of 1 | packaging pk of 20 | packaging pk of 100 |
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Protocols
A simple method to enrich phospholipids from plasma samples, involving a HybridSPE-PPT 96-well plate that both retains phospholipids and removes precipitated proteins.
Articles
This Sigma-Aldrich article continues to detail new methodology for the analysis of Vitamin D metabolites using HybridSPE-Phospholipid technology.
An article focusing on ion-suppression and phospholipid contamination and some of their major causes and difficulties.
This Sigma-Aldrich article continues to detail new methodology for the analysis of Vitamin D metabolites using HybridSPE-Phospholipid technology.
Related Content
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 55269-U | 04061832581996 |