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Enzyme activity assays

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IFN-γ ELISpot Assays on MultiScreen® IP
The ELISpot (Enzyme Linked Immuno-Spot) assay provides an effective method of measuring the antibody or cytokine production of immune cells on the single cell level.
Alkaline Phosphatase (AP) Protocol
Alkaline phosphatase (AP) is a non-specific phosphomonoester hydrolase that catalyzes the hydrolysis of a wide variety of organic monophosphates.
Thrombin from Bovine Plasma
Thrombin is an endolytic serine protease that selectively cleaves the Arg–Gly bonds of fibrinogen to form fibrin and release fibrinopeptides A and B.
Enzymatic Assay of Fumarase
Enzymatic Assay of Fumarase
Enzymatic Assay of L-Lactic Dehydrogenase (EC 1.1.1.27)
This procedure applies to all products from heart muscle that have a specification for L-Lactic Dehydrogenase activity.
Diethanolamine Assay for the Enzymatic Assay of Alkaline Phosphatase (EC 3.1.3.1)
Enzymatic Assay of Alkaline Phosphatase, Diethanolamine Assay (EC 3. 1. 3. 1)
Enzymatic Assay of Carbonic Anhydrase for Wilbur-Anderson Units (EC 4.2.1.1)
Enzymatic Assay of Carbonic Anhydrase for Wilbur-Anderson Units (EC 4.2.1.1)
Procedure for Enzymatic Assay of α-Chymotrypsin (EC 3.4.21.1)
Follow our procedure for the determination of a chymotrypsin activity. This enzymatic assay of alpha chymotrypsin guides you through the entire process and necessary calculations.
Enzymatic Assay of Elastase (EC 3.4.21.36)
Enzymatic Assay of Elastase (EC 3.4.21.36), a continuous spectrophotometric rate determination.
Assay Procedure for Protease
This procedure is for informational purposes including assay Procedure, definition, and calculations for Protease
Enzymatic Assay of Peroxidase (EC 1.11.1.7) 2,2'-Azino-bis(3-Ethylbenzthiazoline-6-Sulfonic Acid) as a Substrate
To standardize a procedure for the assay of Peroxidase using 2,2'-Azino-bis(3-Ethylbenzthiazoline-6-Sulfonic Acid) as a substrate.
Pull-down assays
This page provides information about different pull-down assays for the further isolation of multiprotein complexes to identify their components with products from Cytiva.
Enzymatic Assay of Plasmin with D-Val-Leu-Lys-p-Nitroanilide Dihydrochloride
Protocol for Enzymatic Assay of Plasmin with D-Val-Leu-Lys-p-Nitroanilide Dihydrochloride
Enzymatic Assay of Ferredoxin NADP Reductase (EC 1.18.12)
Enzymatic Assay of Ferredoxin NADP Reductase - To standardize a procedure for the enzymatic assay of Ferredoxin NADP Reductase (EC.1.18.12).
Enzymatic Method for Determining Alanine (Alanine Assay)
Assay protocol for the colorimetric/fluorometric detection of Alanine in biological samples using the alanine assay kit.
Assay Procedure for Xanthine Oxidase Microbial
The appearance of uric acid is measured at 293nm by spectrophotometry.
Spectrophotometric Determination of Reinheitszahl (RZ) for Peroxidase (EC 1.11.1.7)
Reinheitszahl (RZ) is the ratio of absorbance due to hemin (A403, Soret region) to absorbance due to protein (A275).
GST Detection Module with CDNB Enzymatic Assay
This page describes enzymatic detection of GST-tagged proteins with the GST Detection Module from Cytiva.
Enzymatic Assay of Neuraminidase
Enzymatic Assay of Neuraminidase applies to products that have a specification for neuraminidase content by enzymatic determination.
Enzymatic Assay of Diamine Oxidase (E.C. No. 1.4.3.22)
To standardize a procedure for the enzymatic assay of Diamine Oxidase.
Enzymatic Assay of Sulfatase (EC 3.1.6.1.)
Enzymatic Assay of Sulfatase (EC 3.1.6.1.)
Enzymatic Assay of Mutanolysin
This procedure may be used for Mutanolysin products.
Enzymatic Assay of Pectinase
To measure pectinase activity, a titrimetric stop reaction assay is used. One unit of pectinase will liberate 1 μmol of galacturonic acid from poly-galacturonic acid per hour at pH 4.0 at 25 °C.
Enzymatic Assay of Phytase (EC 3.1.3.8)
Derived from procedure SSPHYT02. Includes template updates to current SOP specifications and incorporation of notes into the procedure.
Enzymatic Assay of Amyloglucosidase (EC 3.2.1.3)
This procedure may be used for the determination of Amyloglucosidase activity using starch as the substrate.
Enzymatic Assay of Collagenase (EC 3.4.24.3) using FALGPA (N-(3-[2-Furyl]acryloyl)-Leu-Gly-Pro-Ala)
To measure collagenase activity, N-(3-[2-Furyl]acryloyl)-Leu-Gly-Pro-Ala is used in a continuous spectrophotometric rate determination at 345 nm. Collagenase hydrolyzes collagen peptide bonds.
Enzymatic Assay of 5’-Nucleotidase
To measure 5′-nucleotidase activity, this procedure uses adenosine 5’-monophosphate and a color reagent to create a standard curve for determining the micromoles of phosphorus liberated.
Enzymatic Assay of Hyaluronidase (3.2.1.35)
To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.
Enzymatic Assay: Carboxypeptidase
Carboxypeptidase A activity measured via continuous spectrophotometric rate determination assay with hippuryl-L-phenylalanine substrate.
Enzymatic Assay of Hexokinase
Measure hexokinase activity using a continuous spectrophotometric rate-determination assay at 340 nm, catalyzing D-hexose sugar phosphorylation using ATP.
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