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Applications:Enzyme activity assays

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3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) is a transmembrane glycoprotein, located on the endoplasmic reticulum.

Neurotensin is a 13 amino acid regulatory peptide found mainly in gut and brain. Intestinal neurotensin is produced by a discrete population of endocrine cells (N cells) scattered throughout the jejuno-ileal mucosa.

G Proteins (Heterotrimeric)

Heterotrimeric G proteins, comprising α, β and γ subunits, respond to extracellular signals generated by activated seven-transmembrane (7TM) receptors by modulating intracellular effector proteins such as enzymes and ion channels.

Nuclear Receptors (PPARs)

Peroxisome proliferator activated receptors (PPARs) are members of the nuclear hormone receptor superfamily of ligand-activated transcription factors that are related to retinoid, steroid and thyroid hormone receptors.

Glycobiology - Carbohydrate Enzymes and Proteins

Explore our complex carbohydrate proteins, enzyme products and kits for investigating oligosaccharides, polysaccharides, and numerous additional aspects of your carbohydrate workflow.

Enzymatic Method for Determining Amylase Activity (Amylase Activity Assay)

Assay protocol for the colorimetricdetection of amylase activity in biological samples using the Amylase Activity assay kit.

Enzymatic Assay of Chitosanase from Streptomyces Species

To standardize a procedure for determining the enzymatic activity of Chitosanase.

β Secretase (BACE1) Activity Assay for BACE1 Inhibitor Screening

In recent years, the biological processes involved in the pathogenesis of Alzheimer’s disease (AD) have become increasingly better understood. It is now recognized that a key role is played by BACE1 proteases that cleave amyloid precursor protein (APP) to produce

Protein Kinase C (PKC)

Protein kinase C (PKC) is an AGC kinase that phosphorylates serine and threonine residues in many target proteins.

Bioluminescent Firefly Luciferase Assays

Firefly luciferase is a widely used bioluminescent reporter for studying gene regulation and function. It is a very sensitive genetic reporter due to the absence of endogenous luciferase activity in mammalian cells or tissues.

How to Work with Enzymes Supplied as Ammonium Sulfate Suspensions

Instructions for working with enzymes supplied as ammonium sulfate suspensions

Enzymatic Assay of Cathepsin B

This procedure applies to all products that have a specification for Cathepsin B activity determined by the liberation of 7-amino-4-methylcoumarin from Z-Arg-Arg 7-amido-4-methylcoumarin.

Phosphodiesterases

Cyclic nucleotide phosphodiesterases (PDEs) catalyze the hydrolysis of cAMP and/or cGMP. There are 11 different mammalian PDE families.

Phospholipase D

Phosphatidylcholine (PC)-specific phospholipase D (PLD) enzymes catalyze the hydrolysis of PC to generate choline and phosphatidic acid. These enzymes belong to a superfamily of phosphatidyltransferases with a characteristic unique duplicated catalytic domain motif, the PLD or " HKD"

Phospholipase C

The hydrolysis of a minor membrane phospholipid, phosphatidylinositol 4,5-bisphosphate (PIP2) by a specific phospholipase C (PLC) is one of the earliest key events in the regulation of various cell functions by more than 100 extracellular signaling molecules.

Neuropeptidases

We offers many products related to neuropeptidases for your research needs.

Compound Library Screening with LOPAC®1280 Small Molecules

Learn about compound library screening and explore our compound libraries, including our Library of Pharmacologically Active Compounds (LOPAC®1280) portfolio to experience the gold-standard collection of biologically annotated, ready-to-screen compounds.

Mucins, Glycosylated Proteins, Properties & Diagram

Mucins are a diverse family of densely glycosylated proteins. Mucin domains within the protein core are rich in threonine, serine and hydroxyproline enabling post-translational O-glycosylation.

Enzymatic Method for Determining Branched Chain Amino Acids (BCAA Assay)

Assay protocol for the colorimetric detection of Branched Chain Amino Acids (BCAA) in biological samples using the alanine assay kit.

Horseradish Peroxidase (HRP) Enzymes

Discover our peroxidase from horseradish enzymes, products, substrates, and inhibitors for your ELISA, immunoassay, and protein application needs.

Complement Proteins

Application Index: Complement Proteins offered by Sigma-alderich online.The complement system is a complex cascade involving proteolytic cleavage of serum glycoproteins often activated by cell receptors. This cascade ultimately results in induction of the inflammatory response, phagocyte chemotaxis and opsonization, and

Tubulin

Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells.

Coenzyme A

Enzyme Reagent Coenzyme A (CoA, CoASH or HSCoA) is the key cofactor in first step of the TCA cycle, responsible for transferring the acetyl group from pyruvate oxidation to oxaloacetate yielding citrate. Available through Sigma-Aldrich online.

Cholesterol Esterification

Cholesterol undergoes esterification to improve transport. Cholesterol esters are more easily packaged into the interior of lipoproteins - increasing the quantity that can be readily transported in the blood stream.

Aprotinin

While aprotinin and bovine pancreatic trypsin inhibitor (BPTI) are the same protein sequence, the term aprotinin is typically used when describing the protein derived from bovine lung.