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Gel electrophoresis

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Reproducibility with Biological Buffers
Biological buffers are organic substances that maintain a constant pH over a given range by neutralizing the effects of hydrogen ions.
Selecting DNA, RNA, and PCR Fragment Markers and Ladders for Gel Electrophoresis
Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.
Protein Visualization
Prior to probing a membrane using precious antibodies, it is helpful to visualize the transferred proteins on the blot to ensure complete transfer and even loading. This page describes possible causes and potential remedies for challenges encountered during protein visualization.
Performing a Purity and Homogeneity Check
This page shows how to perform a purification and homogeneity check of membrane proteins with products from Cytiva.
Bis Tris Polyacrylamide Gel Electrophoresis Technology
Bis-Tris gels and buffers for superior protein resolution compared to traditional tris-glycine gels.
Sample Prep & Gel Electrophoresis Troubleshooting
Identify causes and remedies for SDS-PAGE sample preparation challenges and optimize electrophoresis conditions.
Auto2D® Gel Electrophoresis Device
Auto2D® automates difficult 2D electrophoresis methods for protein localization with high reproducibility in under two hours.
Troubleshooting 2-D DIGE Results
Troubleshooting table for 2D DIGE results lists problems, causes, and prevention methods for future experiments.
DNA / Protein Electrophoresis and Troubleshooting Tables
DNA / Protein Electrophoresis and Troubleshooting Tables
Components of Rehydration Solution
This page describes components of rehydration solutions for use in 2-D Electrophoresis with Cytiva products.
Precipitation Procedures
This page describes protein precipitation as an optional step in sample preparation for 2-D electrophoresis with Cytiva products.
Restriction Endonucleases - The Molecular Scissors
The term “Restriction enzyme” originated from the studies of Enterobacteria phage λ (lambda phage) in the laboratories of Werner Arber and Matthew Meselson.
Fluorescent Multiplex Detection using Antibody Atto Dye Conjugates
Immunoblotting (Western blot transfer) is a common technique in modern proteomics research.
mPAGE®Lux Casting System for SDS-PAGE Gels
Discover the mPAGE® Lux Casting System for ready-to-use SDS-PAGE gels in 3 minutes. Fresh SDS-PAGE gels that are ready when you need them.
Protein Staining Reagents Selection Guide
Sigma offers EZBlue™ and ProteoSilver™ reagents for protein visualization, suitable for proteomics and traditional PAGE formats.