Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.
This page discusses the principles and benefits of using ultrafiltration membranes in protein sample preparation. Possible applications include solvent exchange, protein sample concentration, or sample purification.
The Amicon® Stirred Cell family of pressure-driven filtration devices provide an ideal solution for concentrating and buffer-exchanging large volumes of macrosolutes. These devices are available in multiple sizes to offer a wide range of processing volumes.
The correct choice of salt and salt concentration are the most important parameters that influence capacity and final selectivity. The objective is to optimize conditions to achieve the required selectivity to bind the target protein(s) and ensure that the majority