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Protein expression

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Duolink® PLA Brightfield Protocol
Duolink® PLA reagents enable brightfield detection and quantification of proteins and interactions in tissue samples.
Yeast Growth Methods
Yeast culture techniques: Model systems for eukaryotic studies with liquid media or agar plate growth.
Duolink® PLA Multicolor Detection Protocol
Duolink® PLA Multicolor Detection Protocol
Duolink® PLA Probemaker Guide
Guide to create custom PLA probes for immunofluorescent or brightfield detection.
Counterstaining after the Duolink In Situ Protocol
We recommend applying the counterstaining protocol after the completion of the Amplification step in section 7.3, step 5 of the Duolink In Situ Fluorescence User Manual.
Handling Inclusion Bodies in Recombinant Protein Expression
How to isolate proteins from inclusion bodies using Cytiva products.
Duolink® In Situ Short Instructions - Fluorescence
This page details the Duolink® In Situ Short Protocol for fluorescence detection
Duolink® PLA Flow Cytometry Protocol
Protocol for use of Duolink® PLA reagents for the detection of individual proteins, protein modifications, and protein-protein interactions within cell populations by flow cytometry.
Yeast Transformation Protocols
Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells.
Yale Immunofluorescence Protocol
Procedure for Fixation, Immunostaining, and Imaging in 384-well Plates.
Duolink® PLA Fluorescence Protocol
This protocol describes how to perform immunofluorescent detection of proteins in cells and tissue.
ALiCE®Cell-Free Protein Synthesis System Protocol
Achieve high protein yields with the ALiCE® Cell-Free Protein Synthesis System that allows you to obtain “difficult to produce” proteins in a matter of hours instead of weeks.
Next Generation Cell Free Protein Expression Kit (Wheat Germ) (CFPS700) PROTOCOL
Protein synthesis using cell-free protein synthesis reagent kit consists of three stages: preparation of transcription template, transcription and translation.
AliCE® Cell-Free Protein Synthesis Kit Quick Start Guide
Follow these five quick steps to perform cell-free protein synthesis using the ALiCE® protein expression plant lysate kit.
Using Duolink® in Protein Interaction Studies
The video follows the simple and straightforward procedure that allows you to detect, quantify and obtain cell localization of protein interactions and their modifications in a single experiment.
Liberase™ TM Research Grade Protocol & Troubleshooting
Liberase™ TM Research Grade Protocol & Troubleshooting
Measuring Kinase Inhibitor Residence Times
Jump dilution protocol to determine the residence time of a drug (or drug candidate) during its interaction with a kinase using a fluorescence polarization assay based on the detection of ADP.
Creating PLA® Probes
Duolink® kits use in situ PLA® technology for accurate quantification of individual proteins, interactions, and modifications in cells and tissue.