Enzymes provide a non-mechanical method for cell lysis and protoplast preparation. It may seem like a simple process to throw in your enzyme, stick your tube in the water-bath and walk away, but what is actually going on in that
Stabilize complete, uniform tissue homogenates. Maintain RNA and functional proteins with Stabilyser reagent, the only reagent that protects the integrity of nucleic acid and active protein in one uniform lysate mixture.
Protease Inhibitor Cocktails maintain and preserve cellular protein composition following cell lysis. When used with CelLytics, they also preserve target protein activity. These cocktails have been optimized for a wide variety of specific applications, ensuring excellent inhibition and ease of
The isolation of subcellular fractions by centrifugation is a commonly used technique and is widely applicable across multiple cell and tissue types. Because organelles differ in their size, shape, and density, centrifugation can be easily employed to separate and purify
For optimal conditions for growth, induction, and cell lysis of recombinant histidine-tagged proteins, please refer to established procedures. The following is a general procedure for cell lysis and sample preparation from bacterial cultures. Other established procedures may also work.
ReadyShield® phosphatase and protease inhibitor cocktail FAQ for sample protection in a variety of cell types and tissue extracts, including mammalian, plant, and microbial samples. Our ReadyShield® Protease Inhibitor Cocktail is a non-freezing solution that contains inhibitors with a broad
Our yeast cell lysis reagents are versatile, phosphate-free, and non-denaturing. CelLytic Y Cell Lysis reagent provides an effective method for yeast cell lysis and protein solubilization. The CelLytic Y Yeast Enzymatic Lysis Kit provides a convenient method for