Merck

HPLC Analysis of Sugars and Sugar Enantiomers on Astec® CYCLOBOND I 2000

HPLC Analysis of Sugars and Sugar Enantiomers on Astec® CYCLOBOND™ I 2000 application for HPLC

Materials

analytical column

Product No.
Description
Pricing

Astec® CYCLOBOND I 2000 Chiral HPLC Column

5 μm particle size, L × I.D. 25 cm × 4.6 mm

standard

Product No.
Description
Pricing

Sucrose

≥99.5% (GC)

D-(+)-Maltose monohydrate

BioXtra, ≥99%

D-(−)-Fructose

BioXtra

Lactose

tested according to Ph. Eur.

CONDITIONS

column

CYCLOBOND I 2000, 25 cm x 4.6 mm I.D., 5 μm particles (20024AST)

mobile phase

[A] water; [B] acetonitrile; (15:85, A:B)

flow rate

1 mL/min

column temp.

35 °C

detector

ELSD at 35°C and 3.2 bar nitrogen

injection

10 μL

sample

0.5 mg/mL each in water:acetonitrile, 25:75

Description

Legal Information

Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CYCLOBOND is a trademark of Sigma-Aldrich Co. LLC