Syringes are precision instruments. The care you take in using, handling and storing them will dictate their accuracy, reproducibility, and lifetime; also, the quality of the resulting chromatogram can be affected by a damaged syringe.
Routinely inspect the syringe for damage including hairline cracks. Discard any syringe with a potentially unsafe barrel. The needle’s tip must be free of barbs which could potentially rupture the septum and produce particles that might clog the needle, or lodge in the injection port and damage the chromatographic system.
If your syringe contains a PTFE tip, be sure to replace the plunger and tip assembly immediately if any metal is exposed. The exposed metal scrapes the glass of the syringe barrel, producing glass fragments that cut grooves in the PTFE tip. These grooves allow sample to leak past the tip.
Caustic samples or cleaning solutions that seep under the PTFE tip can destroy the sharp barb that holds the tip onto the plunger. The tip, so loosened, could slip off during sample aspiration.
Between samples, the syringe should be flushed 5-10 times its total capacity to eliminate carryover. Draw sample and expel the first several flushes to waste to avoid contamination of the sample.
Never store a syringe without first cleaning it and then wiping dry all external surfaces.
A syringe should be flushed with approximately 5-10 times its total capacity to eliminate carryover between samples. This is achieved by repeatedly drawing and expelling solvent/sample from the syringe. To avoid contaminating the sample, the first 2-3 washes should be discarded to waste.
Syringes do not require any lubricating grease. Grease may cause a variety of problems including: sample cross contamination, seizing of the plunger in the barrel or barrel damage. Minimize the use of a dry syringe; for lubrication, use an appropriate solvent that is compatible with the sample.
Grasp the syringe only by the flange and plunger button. By doing so, variations in liquid measurement due to body heat are avoided.
Pump the plunger with the syringe needle immersed in the fluid to be transferred to expel any trapped air in the needle and syringe.
If the plunger is accidentally removed from the syringe barrel, wipe it carefully with a lint-free tissue. Reinsert the plunger into the barrel and pump deionized water or acetone through the needle and syringe. In the case of plungers with PTFE tips (like Hamilton’s Gastight syringes), re-wet the PTFE plunger tip prior to reinserting it into the barrel. Avoid touching the exposed plunger since any abrasions, scratches, or oil from one’s fingers will often interfere with proper plunger operation.
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