Merck
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1.09261

Sigma-Aldrich

Lugol′s solution (diluted iodine-potassium iodide solution)

for the Gram staining method

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别名:
Iodine-potassium iodide solution

质量水平

形式

liquid

IVD

for in vitro diagnostic use

pH值(酸碱度)

3.5 (20 °C in H2O)

bp

100 °C/1013 hPa

密度

1.01 g/cm3 at 20 °C

应用

clinical testing
diagnostic assay manufacturing
hematology
histology

储存温度

15-25°C

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此商品
3292262650L6146
Lugol 溶液 according to Lugol

32922

Lugol 溶液

Lugol 溶液 for microscopy (Bact., Bot.)

62650

Lugol 溶液

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L6146

Lugol 溶液

application(s)

clinical testing
diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
food and beverages
hematology
histology

application(s)

-

application(s)

-

IVD

for in vitro diagnostic use

IVD

-

IVD

-

IVD

-

storage temp.

15-25°C

storage temp.

room temp

storage temp.

-

storage temp.

-

Quality Level

400

Quality Level

200

Quality Level

200

Quality Level

200

density

1.01 g/cm3 at 20 °C

density

1.006 g/cm3 at 20 °C

density

1.007 g/mL at 20 °C

density

-

一般描述

Lugol′s solution (diluted iodine-potassium iodide solution - for the Gram staining method, is a ready-to-use solution used in Bacteriology, for example, for the staining of Gram bacteria. In bacteriology, the Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative. The mureine structure of the bacteria wall is the basis of the color affinity. In the first step, bacteria will be stained with crystal violet (Product number 1.09218), an aniline dye. After the treatment with iodine solution (Lugol′s solution), a dye-iodine complex will form. During the decolorizing step (Gram′s decolorizing solution, Product number 1.10218), this complex stays in the multilayer mureine structure of the cell wall of Gram-positive bacteria - they will appear blue-violet. Gram-negative bacteria, by contrast, have a cell wall consisting of a single-layered murein structure, and correspondingly re-release the staining dye with the decoloring solution. Gram-negative bacteria will be counterstained with safranine solution (Product number 1.09217) and will then appear pink to red.
Lugol′s solution (diluted iodine-potassium iodide solution) is capable of staining up to 500 slides. This product is registered as IVD and CE marked. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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