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Merck

XTGHP-RO

Roche

X-tremeGENE HP DNA转染试剂

High-performance polymer reagent for transfecting many cell lines

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About This Item

分類程式碼代碼:
41106502
NACRES:
NA.55

等級

for molecular biology

品質等級

形狀

liquid (aqueous solution)

用途

 mL (suitable for 165 transfections)

包裝

pkg of 0.4 mL (06366244001)
pkg of 1.0 mL (06366236001)
pkg of 5 × 1 mL (06366546001)

製造商/商標名

Roche

技術

transfection: suitable

儲存溫度

−20°C

一般說明

X-tremeGENE HP DNA转染试剂是一款多组分试剂,能够与DNA形成复合物,并将其递送至动物或昆虫细胞中。

特點和優勢

X-tremeGENE HP DNA转染试剂是一种高效转染试剂,可成功转染多种真核细胞,包括昆虫细胞和难转染细胞系(如Sf9、U-2 OS、MEF、MCF7、Hep G2、PC-3、HeLa、HT-29、HT-1080、K-562、RAW 264.7、Jurkat、PC-12和HCT 116)。

  • 简单易用的非脂质体试剂 无动物源性成分,室温稳定,经0.2 μm滤膜过滤,并且在有血清培养液中仍保持活性。
  • 高转染效率 对于原代细胞和使用其它试剂难转染的肿瘤细胞系有高转染效率。
  • 结果生理相关性好 使用细胞毒性低的试剂。
  • 满足高通量实验 实现简单一致的靶标评估。

品質

每一批次的X-tremeGENE HP DNA转染试剂都经过严格的质量控制流程测试,确保性能合规的标准性。在质量测试环节,首先使用X-tremeGENE HP DNA转染试剂(试剂DNA比为3:1μl/μg DNA)向细胞转染一个报告基因载体。再通过化学荧光检测法监测报告基因活性。最后使用一条标准曲线对每孔的重组蛋白总量进行定量,以确定产品达到规格。

外觀

本产品溶于80%乙醇中,经0.2 μm滤膜过滤。无人源或动物源成分。次数:使用标准步骤时,在96孔板内,1ml X-tremeGENE™ HP DNA转染试剂可以进行10,000次转染。

其他說明

仅用于生命科学研究。不可用于诊断。

法律資訊

X-tremeGENE is a trademark of Roche

象形圖

FlameExclamation mark

訊號詞

Danger

危險聲明

危險分類

Eye Irrit. 2 - Flam. Liq. 2

儲存類別代碼

3 - Flammable liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

334.4 °F

閃點(°C)

168 °C


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转染试剂I

Sigma-Aldrich

Sigma-Aldrich

T1706

TransIT-CRISPR®

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A synthetic analog of sphingosine named FTY720 (Fingolimod), phosphorylated by sphingosine kinase-2, interacts with sphingosine-1-phosphate (S1P) receptors expressed on various cells. FTY720 suppresses the disease activity of multiple sclerosis (MS) chiefly by inhibiting S1P-dependent egress of autoreactive T lymphocytes from
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商品

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实验方案

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