This buffer is comprised of 10 mM Tris-HCl and 1 mM disodium EDTA, pH 8.0.
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等級
Molecular Biology
品質等級
產品線
BioUltra
形狀
liquid
雜質
DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected
pH值
8.0±0.2 (25 °C)
正離子痕跡
Al: ≤1 mg/kg
Ba: ≤1 mg/kg
Bi: ≤1 mg/kg
Ca: ≤5 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤1 mg/kg
Fe: ≤1 mg/kg
K: ≤20 mg/kg
Li: ≤1 mg/kg
Mg: ≤1 mg/kg
Mn: ≤1 mg/kg
Mo: ≤1 mg/kg
Ni: ≤1 mg/kg
Pb: ≤1 mg/kg
Sr: ≤1 mg/kg
Zn: ≤1 mg/kg
&lambda ；
neat
紫外吸收
λ: 260 nm Amax: 0.04
λ: 280 nm Amax: 0.01
應用
sample preparation
SMILES 字串
NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
InChI
1S/C10H16N2O8.C4H11NO3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2
InChI 密鑰
VLEIUWBSEKKKFX-UHFFFAOYSA-N
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一般說明
Tris EDTA是生物学研究中的一种缓冲液。它是Tris碱和EDTA（一种金属螯合剂）的组合。Tris提供缓冲能力，可在生物样本中维持稳定的pH值，而EDTA则通过螯合二价金属离子来防止金属离子的干扰。Tris EDTA常用于分子生物学技术中，如DNA和RNA样本制备、电泳和酶促反应。它通过抑制核酸酶和金属依赖性酶的活性，帮助维持核酸的稳定性和完整性。
應用
TE缓冲液在一般提取、悬浮、洗涤和储存缓冲液以及DNA或RNA的制备中很有用。它通常用于DNA测序、限制性酶消化、连接和聚合酶链式反应（PCR）等应用。
特點和優勢
- BioUltra级别 - 适用于许多分子生物学应用
- 无DNase、RNase、磷酸酶和蛋白酶
- 高纯度，无微量金属。
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
nwg
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves
Marina Ishii et al.
Applied biochemistry and biotechnology, 137-140(1-12), 555-571 (2008-05-15)
The thermal stability of recombinant green fluorescent protein (GFP) in sodium chloride (NaCl) solutions at different concentrations, pH, and temperatures was evaluated by assaying the loss of fluorescence intensity as a measure of denaturation. GFP, extracted from Escherichia coli cells
Quan-Ping Su et al.
Vaccine, 25(6), 970-975 (2006-10-24)
We have demonstrated that phage display Candida albicans (C. albicans) LKVIRK epitope was protective in systemically infected C57BL/6J mice. The different development from precursor Ths, Th1 or Th2, will result in a protective or nonprotective immune response. To compare the
A A Khan et al.
Journal of industrial microbiology & biotechnology, 20(2), 90-94 (1998-06-27)
A rapid and sensitive method for the detection of genetically engineered microorganisms in soil and sediments has been devised by in vitro amplification of the target DNAs by a polymerase chain reaction. A cloned catechol 2,3-dioxygenase gene located on the
Stuart W Tompson et al.
Bio-protocol, 7(10) (2017-08-02)
There are several in silico programs that endeavor to predict the functional impact of an individual's sequence variation at splice donor/acceptor sites, but experimental confirmation is problematic without a source of RNA from the individual that carries the variant. With
Inbar Ben-Zvi et al.
ESC heart failure, 7(3), 835-843 (2020-04-08)
The aims of the study are to assess the levels of coronary sinus (CS) miRNAs of systolic heart failure (HF) patients in samples obtained during cardiac resynchronization therapy (CRT) device implantation and compare them to the peripheral systemic venous miRNA
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Is this buffer composed by 10mM Tris and 1 mM EDTA?
1 answer-
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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How can I determine the shelf life / expiration / retest date of this product?
1 answer-
If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdfHelpful?
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What is the storage condition for TE buffer BioUltra
1 answer-
This product may be stored at room temperature.
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What is the concentration of Tris and EDTA in this buffer?
1 answer-
This buffer is 10 mM Tris-HCl, 1 mM disodium EDTA, pH 8.0.
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