所有图片(1)

X100

Sigma-Aldrich

Triton X-100

laboratory grade

别名:
4-(1,1,3,3-四甲基丁基)苯基-聚乙二醇, 叔辛基苯氧基聚乙烯乙氧基乙醇, 聚乙二醇叔辛基苯基醚
线性分子式:
t-Oct-C6H4-(OCH2CH2)xOH, x= 9-10
CAS号:
Beilstein:
2315025
MDL编号:
PubChem化学物质编号:
NACRES:
NA.21

质量水平

200

等级

laboratory grade

描述

non-ionic

形式

liquid

分子量

micellar avg mol wt 80,000
average mol wt 625

聚集数

100-155

CMC

0.2-0.9 mM (20-25°C)

转变温度

cloud point 65 °C
pour point ~7 °C

溶解性

water: 0.1 mL/mL, clear to slightly hazy, colorless to faintly yellow

HLB

13.5

SMILES string

CC(C)(C)CC(C)(C)c1ccc(OCCOCCOCCOCCOCCOCCOCCO)cc1

InChI

1S/C28H50O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h6-9,29H,10-24H2,1-5H3

InChI key

HNLXNOZHXNSSPN-UHFFFAOYSA-N

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相关类别

一般描述

Triton X-100具有溶血作用,可用于DNA提取。
Triton X-100是一种常用的非离子型的表面活性剂和乳化剂,经常用于生化应用以溶解蛋白质。它被视为一种不变性的相对温和的洗涤剂,并且在许多参考文献中作为一种常规被添加的试剂而被报道。它用于裂解细胞以提取蛋白和细胞器。还可通透活细胞膜用于转染。

应用

Triton-X已用于:
  • 细胞通透化处理用于免疫荧光染色
  • western blot分析中裂解缓冲液中的一种成分
  • 制备免疫金标记的细胞切片用于电子显微镜的Tris盐缓冲液中的一种成分

包装

5 mL in glass bottle
1 gal in poly bottle
5 gal in poly drum
1 L in poly bottle
100, 500 mL in poly bottle

生化/生理作用

广泛使用的非离子表面活性剂,用于在温和的非变性条件下进行膜恢复。

法律信息

Tri-Chek is a trademark of Micro Essential Laboratory, Inc.
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

警示用语:

Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2

Storage Class Code

10 - Combustible liquids

WGK Germany

WGK 3

闪点(F)

483.8 °F

闪点(C)

251 °C

个人防护装备

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter, type N95 (US)

分析证书

原产地证书 (CofO)

  1. What is the molecular weight of X100, Triton® X-100?

    Based on an average of 9-10 ethylene oxide units per molecule, the molecular weight of Triton X-100 is 625 g/mol.

  2. What is the concentration of X100, Triton® X-100?

    This product is 100% active ingredient.

  3. What concentration of X100,Triton® X-100, is typically used to lyse cells?

    Typically, a 0.1 % solution of Triton X-100 is sufficient for lysing cells.

  4. Does X100, Triton® X-100, have any UV absorbance?

    X100 does have UV absorbance at 280 nm, which will interfere with the UV determination of proteins.

  5. Can I autoclave X100, Triton® X-100?

    Aqueous solutions of Triton X-100 are stable to autoclaving. At certain concentrations the solutions may be cloudy but dispersible above the cloud point. However, the solution should clear with stirring upon cooling.

  6. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  7. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Growth and hydration of Triton X-100 micelles in monovalent alkali salts: a light scattering study
Molina-Bolivar JA, et al.
The Journal of Physical Chemistry B, 106(4), 870-877 (2002)
Masoumeh Zangeneh et al.
Materials science & engineering. C, Materials for biological applications, 103, 109739-109739 (2019-07-28)
The purpose of this study was to investigate the radiation dose enhancement effects of gadolinium-doped zinc oxide nanoparticles (Gd-doped ZnO NPs) under the megavoltage (MV) X-ray irradiation. ZnO NPs have preferred photocatalytic properties under UV light for cancer killing. UV...
Knockdown of miR-210 decreases hypoxic glioma stem cells stemness and radioresistance
Yang W, et al.
Experimental Cell Research, 326(1), 22-35 (2003)
José Francisco Lima-Barbero et al.
Experimental & applied acarology, 78(4), 555-564 (2019-08-02)
Blood-feeding ectoparasites constitute a growing burden for human and animal health, and animal production worldwide. In particular, mites (Acari: Gamasida) of the genera Dermanyssus (Dermanyssidae) and Ornithonyssus (Macronyssidae) infest birds and cause gamasoidosis in humans. The tropical fowl mite, Ornithonyssus...
alpha -Synuclein Is Degraded by Both Autophagy and the Proteasome
Webb LJ, et al.
The Journal of Biological Chemistry, 278(27), 25009-25013 (2003)

实验方案

Enzymatic Assay of Cholesterol Oxidase

This procedure applies to products that have a specification for the enzymatic activity of cholesterol oxidase. This assay is NOT to be used to assay Cholesterol Oxidase from Schizophyllum commune (Discontinued Product Number C7274) and from Brevibacterium sp. (Discontinued Product Number C8153).

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The liver excretes excess cholesterol in the form of bile acids. Bile acids serve two purposes: to remove unwanted cholesterol from the body and to aid in lipid digestion in the intestine.

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