Merck

32922

Sigma-Aldrich

Lugol 溶液

according to Lugol

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别名:
碘/碘化钾溶液
MDL编号:
PubChem化学物质编号:
NACRES:
MA.02

形式

liquid

质量水平

质量

according to Lugol

密度

1.006 g/cm3 at 20 °C

application(s)

diagnostic assay manufacturing
food and beverages
hematology
histology

储存温度

room temp

SMILES string

[K+].[I-].II

InChI

1S/I2.HI.K/c1-2;;/h;1H;/q;;+1/p-1

InChI key

XUEKKCJEQVGHQZ-UHFFFAOYSA-M

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此商品
L6146626501.09261
vibrant-m

32922

Lugol 溶液

vibrant-m

L6146

Lugol 溶液

vibrant-m

62650

Lugol 溶液

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

400

storage temp.

room temp

storage temp.

-

storage temp.

-

storage temp.

15-25°C

density

1.006 g/cm3 at 20 °C

density

-

density

1.007 g/mL at 20 °C

density

1.01 g/cm3 at 20 °C

application(s)

diagnostic assay manufacturing
food and beverages
hematology
histology

application(s)

-

application(s)

-

application(s)

clinical testing
diagnostic assay manufacturing
hematology
histology

quality

according to Lugol

quality

-

quality

-

quality

-

一般描述

Lugol′s solution is a ready-to-use solution that comprises 6% iodine in 4% potassium iodide. It is commonly used as a mordant for Gram staining in bacteriology. It is used for staining bacterial target structures for diagnostic purposes by fixing, staining, counterstaining, and mounting bacteriological specimen materials.

应用

  • Lugol′s solution is generally used as a mordant in Gram staining, and for fixing invertebrate blood cells and protozoa.
  • It is suitable for the detection of starch in plants.
  • It has been used in a study to assess narrow-band imaging without image magnification for detecting high-grade dysplasia and intramucosal esophageal squamous cell carcinoma.
  • It has been used in a study to determine its benefit in aiding the detection, presence, and spread of small squamous cell carcinomas of the esophagus.

生化/生理作用

Iodine diffuses rapidly through hydrophilic and hydrophobic domains of tissues and loosely binds to proteins, staining them brown. It reacts irreversibly with unsaturated lipid linkages and with tyrosine and histidine aromatic rings.

特点和优势

  • Ready-to-use solution.

原理

In bacteriology, Gram staining allows rapid differentiation between Gram-positive and Gram-negative bacteria. The mureine structure of the bacterial cell wall is the basis of the color affinity. Bacteria are first stained with the primary stain, crystal violet. Lugol solution is added thereafter as the mordant, forming crystal violet-iodine complexes. During the decolorization, these complexes stay entrapped in the multilayer mureine structure of the cell wall of Gram-positive bacteria which appear blue-violet. Gram-negative bacteria have a single-layered cell wall that does not retain the dye-iodine complexes and are counterstained with safranine solution, appearing pink.

储存分类代码

10 - Combustible liquids

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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Histological and Histochemical Methods: Theory and Practice
J.A. Kiernan
Histological and Histochemical Methods: Theory and Practice (2015)
Jason M Cole et al.
Proceedings of the National Academy of Sciences of the United States of America, 115(25), 6335-6340 (2018-06-07)
In the field of X-ray microcomputed tomography (μCT) there is a growing need to reduce acquisition times at high spatial resolution (approximate micrometers) to facilitate in vivo and high-throughput operations. The state of the art represented by synchrotron light sources

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